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Thermo Scientific™ SuperSignal™ West Pico PLUS and SuperSignal™ West Femto Sample

Provides chemiluminescent detection substrates for both routine and high sensitivity western blots

Supplier:  Thermo Scientific™ A43840


Catalog No. PIA43840

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SuperSignal™ West Pico PLUS:
  • SuperSignal™ West Pico PLUS Chemiluminescent Substrate enables picogram to high femtogram-level protein detection by western blot analysis
  • It is best for routine, everyday western blotting applications, providing excellent signal intensity, sensitivity and duration across a wide range of targets
  • Sensitivity: pictogram to femtogram sensitivity
  • Stability: 8-hr working solution stability; 1-year kit stability at room temperature
  • Compatibility: nitrocellulose and PVDF membranes
  • Signal duration: 6 to 24 hours
  • Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–1.0 µg/mL)
  • Recommended secondary antibody concentration: 1:20,000–100,000 dilution (10–50 ng/mL)

SuperSignal™ West Femto:

  • SuperSignal™ West Femto Chemiluminescent Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for low femtogram protein level detection by western blot analysis with horseradish peroxidase (HRP) enzyme
  • It is optimized to detect low abundant and/or difficult to detect targets
  • Sensitivity: low-femtogram (mid-zeptomole)
  • Stability: 8-hr working solution stability, 1-year kit stability at 4°C
  • Compatibility: nitrocellulose and PVDF membranes
  • Signal duration: up to 8 hours
  • Recommended primary antibody concentration: 1:5,000–1:100,000 dilution (10–200 ng/mL)
  • Recommended secondary antibody concentration: 1:100,000–1:500,000 dilution (2–10 ng/mL)


HRP Substrate
Western Blot
500 mL plus 20 mL
Room Temperature and 4°C


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