Thermo Scientific™ SuperSignal™ West Pico PLUS and SuperSignal™ West Femto Sample

Description

Provides chemiluminescent detection substrates for both routine and high sensitivity western blots

SuperSignal™ West Pico PLUS:

• SuperSignal™ West Pico PLUS Chemiluminescent Substrate enables picogram to high femtogram-level protein detection by western blot analysis
• It is best for routine, everyday western blotting applications, providing excellent signal intensity, sensitivity and duration across a wide range of targets
• Sensitivity: pictogram to femtogram sensitivity
• Stability: 8-hr working solution stability; 1-year kit stability at room temperature
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: 6 to 24 hours
• Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–1.0 μg/mL)
• Recommended secondary antibody concentration: 1:20,000–100,000 dilution (10–50 ng/mL)

SuperSignal™ West Femto:

• SuperSignal™ West Femto Chemiluminescent Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for low femtogram protein level detection by western blot analysis with horseradish peroxidase (HRP) enzyme
• It is optimized to detect low abundant and/or difficult to detect targets
• Sensitivity: low-femtogram (mid-zeptomole)
• Stability: 8-hr working solution stability, 1-year kit stability at 4°C
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: up to 8 hours
• Recommended primary antibody concentration: 1:5,000–1:100,000 dilution (10–200 ng/mL)
• Recommended secondary antibody concentration: 1:100,000–1:500,000 dilution (2–10 ng/mL)

Specifications

• Product Type: HRP Substrate
• For Use With (Application): Western Blot
• Quantity: 500 mL plus 20 mL
• Content And Storage: Room Temperature and 4°C

Safety and Handling

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