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Thermo Scientific™ SuperSignal™ West Pico PLUS and SuperSignal™ West Femto Sample
Provides chemiluminescent detection substrates for both routine and high sensitivity western blots
Supplier: Thermo Scientific™ A43840

Description
SuperSignal™ West Pico PLUS:- SuperSignal™ West Pico PLUS Chemiluminescent Substrate enables picogram to high femtogram-level protein detection by western blot analysis
- It is best for routine, everyday western blotting applications, providing excellent signal intensity, sensitivity and duration across a wide range of targets
- Sensitivity: pictogram to femtogram sensitivity
- Stability: 8-hr working solution stability; 1-year kit stability at room temperature
- Compatibility: nitrocellulose and PVDF membranes
- Signal duration: 6 to 24 hours
- Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–1.0 µg/mL)
- Recommended secondary antibody concentration: 1:20,000–100,000 dilution (10–50 ng/mL)
SuperSignal™ West Femto:
- SuperSignal™ West Femto Chemiluminescent Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for low femtogram protein level detection by western blot analysis with horseradish peroxidase (HRP) enzyme
- It is optimized to detect low abundant and/or difficult to detect targets
- Sensitivity: low-femtogram (mid-zeptomole)
- Stability: 8-hr working solution stability, 1-year kit stability at 4°C
- Compatibility: nitrocellulose and PVDF membranes
- Signal duration: up to 8 hours
- Recommended primary antibody concentration: 1:5,000–1:100,000 dilution (10–200 ng/mL)
- Recommended secondary antibody concentration: 1:100,000–1:500,000 dilution (2–10 ng/mL)
Specifications
HRP Substrate | |
Western Blot | |
500 mL plus 20 mL | |
Room Temperature and 4°C |
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