Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate

Catalog No.	Quantity
FER34580X4	4 x 500 mL
PI34579	20 mL
PI34577	200 mL
PI34580	500 mL
PI34578	1 L
FER34578X4	4 x 1 L

Catalog No. FER34580X4 Supplier Thermo Scientific™ Supplier No. 34580X4

Description

Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.

Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.

SuperSignal West Pico PLUS Chemiluminescent Substrate characteristics include:

• Sensitivity: picogram to femtogram sensitivity
• Stability: 8-hr working solution stability; 1-year kit stability at room temperature
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: 6 to 24 hours
• Recommended primary antibody concentration: 1:1000–1:5000 dilution (0.2–1.0 μg/mL)
• Recommended secondary antibody concentration: 1:20,000–100,000 dilution (10–50 ng/mL)

SuperSignal West Pico PLUS substrate is designed to provide excellent signal intensity and sensitivity for western blotting with HRP conjugates. The intensity of the light emission combined with the exceptional duration allows for the acquisition of multiple exposures to more easily obtain publication-quality blot images. SuperSignal West Pico PLUS substrate is compatible with different membranes, blocking reagents, and a wide range of antibody dilutions, making it an ideal choice for most western blotting applications.

Specifications

• Product Type: Substrate
• Detection Method: Chemiluminescence
• Form: Liquid
• For Use With (Application): Western Blot
• Product Line: SuperSignal
• Quantity: 4 x 500 mL
• Conjugate: HRP
• Substrate: Horseradish Peroxidase
• Recommended Antibody Concentrations: 2°: 1:20-100 K (10-50 ng/mL), 1°: 1:1 K (0.2-1 μg/mL)
• Sensitivity: Low picogram to high femtogram
• Signal Duration: 6 to 24 hr.
• Kit Contents: Luminol/Enhancer, 4 x 250 mL, Stable Peroxide Buffer, 4 x 250 mL
• Content And Storage: • SuperSignal West Pico PLUS Luminol/Enhancer, 4 x 500 mL
  
  Store at room temperature.
• Shipping Condition: Room Temperature

Frequently Asked Questions (FAQs)

How do I make the working solution for the SuperSignal West Pico PLUS Chemiluminescent Substrate?

To prepare the working solution for the SuperSignal West Pico PLUS Chemiluminescent Substrate, follow these steps:

- Mix equal parts of the SuperSignal West Pico PLUS Luminol/Enhancer Solution and SuperSignal West Pico PLUS Stable Peroxide Solution. For example, for a mini blot, mix 5 mL of SuperSignal West Pico PLUS Luminol/Enhancer Solution with 5 mL of SuperSignal West Pico PLUS Stable Peroxide Solution.
- Ensure that the volume is sufficient to completely wet the blot and prevent it from drying out. The recommended volume is 0.1 mL/cm2.
- Note that the working solution is stable for up to 8 hours at room temperature. Avoid exposure to the sun or other intense light. Short-term exposure to lab lighting is okay.

For Research Use Only. Not for use in diagnostic procedures.