Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate

Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.

Supplier:  Thermo Scientific™ 34577

Catalog No. PI34577

Description

Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.

SuperSignal West Pico PLUS Chemiluminescent Substrate characteristics include:

• Sensitivity: pictogram to femtogram sensitivity
• Stability: 8-hr working solution stability; 1-year kit stability at room temperature
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: 6 to 24 hours
• Recommended primary antibody concentration: 1:1000–1:5000 dilution (0.2–1.0 μg/mL)
• Recommended secondary antibody concentration: 1:20,000–100,000 dilution (10–50 ng/mL)

SuperSignal West Pico PLUS substrate is designed to provide excellent signal intensity and sensitivity for western blotting with HRP conjugates. The intensity of the light emission combined with the exceptional duration allows for the acquisition of multiple exposures to more easily obtain publication-quality blot images. SuperSignal West Pico PLUS substrate is compatible with different membranes, blocking reagents, and a wide range of antibody dilutions, making it an ideal choice for most western blotting applications.

Specifications

• Product Type: Substrate
• Form: Liquid
• Membrane Compatibility: Nitrocellulose, PVDF
• Quantity: 200 mL
• Substrate: Horseradish Peroxidase
• Sensitivity: Picogram to high femtogram-level
• Sufficient For: 25 Mini-Gel Blots, 2000 cm² of Membrane
• Content And Storage: Sufficient for 25 mini-gel blots; 2000 cm² of membrane
  • SuperSignal West Pico PLUS Luminol/Enhancer, 100 mL
  • SuperSignal West Pico PLUS Stable Peroxide, 100 mL
  
  Store at room temperature.

• Detection Method: Chemiluminescence
• For Use With (Application): Western Blot
• Product Line: SuperSignal™
• Conjugate: HRP
• Recommended Antibody Concentrations: 2°: 1:20-100 K (10-50 ng/mL), 1°: 1:1 K (0.2-1 μg/mL)
• Signal Duration: 6 to 24 hr.
• Kit Contents: SuperSignal West Pico PLUS Luminol/Enhancer, SuperSignal West Pico PLUS Stable Peroxide
• Shipping Condition: Room Temperature

For Research Use Only. Not for use in diagnostic procedures.