Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate
An enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by western blot analysis
Manufacturer: Thermo Scientific™ 34580
DescriptionThermo Scientific™ SuperSignal™ West Pico PLUS substrate is designed to provide excellent signal intensity and sensitivity for western blotting with HRP conjugates. The intensity of the light emission combined with the exceptional duration allows for the acquisition of multiple exposures to more easily obtain publication-quality blot images. SuperSignal West Pico PLUS substrate is compatible with different membranes, blocking reagents, and a wide range of antibody dilutions making it an ideal choice for most western blotting applications.
Features of SuperSignal West Pico PLUS Chemiluminescent Substrate:
- Picogram to femtogram sensitivity—detect low-picogram to high-femtogram amounts of target protein on nitrocellulose or PVDF membrane
- High signal stability—incubated blots provide stable signal duration over the critical 4 hour time period with up to 24 hours of light output under optimal conditions
- Stable reagent—8-hour working solution stability; 1-year kit stability at room temperature
- Economical—optimized for dilute antibody concentrations:
- 0.2 to 1.0µg/mL primary antibody (1:1000 to 1:5000 dilution from 1mg/mL stock)
- 10 to 50ng/mL secondary antibody (1:20,000 to 1:100,000 dilution from 1mg/mL stock)
- Exceptional robustness—provides high performance outside of the recommended antibody dilution ranges, including the most common 1:5K to 1:10K secondary antibody dilutions of a 1mg/mL stock solution
For Research Use Only. Not for use in diagnostic procedures.
This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.
|HRP (Horseradish Peroxidase) Substrate|
|SuperSignal West Pico PLUS Luminol/Enhancer, 250mL, SuperSignal West Pico PLUS Stable Peroxide, 250mL|
We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.
Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.Ok