Thermo Scientific™ SuperSignal™ Western Blot Enhancer

Catalog No.	Quantity
PI46641	50 mL
PI46640	500 mL

Catalog No. PI46641 Supplier Thermo Scientific™ Supplier No. 46641

Description

Increase both signal intensity and sensitivity 3- to 10-fold with this membrane treatment reagent and primary antibody diluent.

Thermo Scientific™ SuperSignal Western Blot Enhancer contains a membrane treatment reagent and a primary antibody diluent that increase both signal intensity and sensitivity 3- to 10-fold compared to detection performed without it.

When a protein or antigen is difficult to detect because of low abundance or poor immunoreactivity, use of SuperSignal Western Blot Enhancer can significantly reduce background and enhance detection of low-abundance and weakly immunoreactive antigens. This versatile kit works with PVDF and nitrocellulose membranes and is compatible with fluorescent, chromogenic and chemiluminescent detection.

Highlights:

• Increase sensitivity – achieve 3- to 10-fold increase in signal intensity and sensitivity

• Improve specificity – improves signal-to-noise ratio for poor quality and low affinity antibodies

• Better clarity – reduces background for cleaner Western blots

• Membrane compatibility – provides effective signal enhancement with PVDF and nitrocellulose membranes

• Substrate compatibility – validated for use with chromogenic, chemiluminescent and fluorescent detection methods

Specifications

• Product Type: Western Blot Enhancer
• Detection Method: Chemiluminescence, Fluorescence, Colorimetric
• Form: Liquid
• For Use With (Application): Western Blot
• Membrane Compatibility: Nitrocellulose, PVDF
• Product Line: SuperSignal
• Quantity: 50 mL
• Content And Storage: Upon receipt store solutions at 4°C Products are shipped at ambient temperature.

Frequently Asked Questions (FAQs)

How can I reduce background with SuperSignal West Atto Ultimate Sensitivity Substrate?

Here are potential causes for high background and corresponding solutions:
- Too much HRP in the system: Further dilute the HRP-conjugate, see guidelines in Table 1 in the user guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0018858_SuperSignalWestAtto_UG.pdf).
- Inadequate blocking: Optimize blocking conditions.
- Inappropriate blocking reagent: Try a different blocking reagent. Use clear milk or StartingBlock blocking buffer instead of dry milk.
- Inadequate washing: Increase length, number or volume of washes.
- Film has been overexposed: Decrease exposure time or use Pierce Background Eliminator (Cat No. 21065).
- Concentration of antigen or antibody is too high: Decrease amount of antigen or antibody.
- Poor antibody specificity: Use SuperSignal Western Blot Enhancer (Cat. No. 46640).
We also recommend minimizing handling of the membrane, using clean gloves while handling, clean plasticware, glassware, forceps, or other contact surfaces. We also recommend trimming of the membrane to remove excess area.

Do I have to use a SuperSignal Western Blot substrate after using the SuperSignal Western Blot Enhancer?

Not necessarily. The SuperSignal Western Blot Enhancer will work with any chemiluminescent substrate (including SuperSignal Western Blot substrates), as well as colorimetric or fluorometric detection systems.

What is the difference between Western Blot Signal Enhancer and SuperSignal Western Blot Enhancer? Is there any benefit to one versus the other?

The reagents in both kits are different. Western Blot Signal Enhancer (Cat. No. 21050) is a 2-step pre-treatment that occurs before blocking. It basically relaxes the protein on the membrane making the epitope easier to detect. SuperSignal Western Blot Enhancer (Cat. Nos. 46640, 46641) contains a membrane treatment reagent and a primary antibody diluent that increase both signal intensity and sensitivity 3- to 10-fold compared to a detecion performed without it. We have found that SuperSignal Western Blot Enhancer shows much less background while enhancing signal and sensitivity when compared to Western Blot Signal Enhancer.

When should I use the SuperSignal Western Blot Enhancer (Cat. Nos. 46640 and 46641)?

The SuperSignal Western Blot Enhancer kit contains two reagents:

- Antigen Pretreatment Solution: used after transferring proteins to the membrane and before blocking or staining the membrane
- The Primary Antibody Diluent: used to dilute the primary antibody

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.