SUPT6H Rabbit anti-Human, Mouse, Polyclonal, Bethyl Laboratories

Rabbit Polyclonal Antibody

Supplier:  Bethyl Laboratories, Inc A300802A

Catalog No. 50-155-8482

Description

The recommended shelf life for this product is 1 year from date of receipt. Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Based on 100% sequence identity, this antibody is predicted to react with D. melanogaster and Zebrafish

Transcription elongation factor which binds histone H3 and plays a key role in the regulation of transcription elongation and mRNA processing. Enhances the transcription elongation by RNA polymerase II (RNAPII) and is also required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. Besides chaperoning histones in transcription, acts to transport and splice mRNA by forming a complex with IWS1 and the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2), to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription. SUPT6H via its association with SETD1A, regulates both class-switch recombination and somatic hypermutation through formation of H3K4me3 epigenetic marks on activation-induced cytidine deaminase (AICDA) target loci. Promotes the activation of the myogenic gene program by entailing erasure of the repressive H3K27me3 epigenetic mark through stabilization of the chromatin interaction of the H3K27 demethylase KDM6A.

Specifications

• Antigen: SUPT6H
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: SUPT6H
• Gene Alias: KIAA0162, MGC87943, SPT6, SPT6H, emb-5, hSPT6; tat-CT2 protein; tat-cotransactivator 2 protein; transcription elongation factor SPT6
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 20926, 6830
• Content And Storage: 4° C
• Form: Liquid

• Applications: Immunohistochemistry, Western Blot
• Concentration: 0.2 mg/ml
• Formulation: TBS with 0.1% BSA and 0.09% sodium azide
• Gene Accession No.: Q62383, Q7KZ85
• Gene Symbols: Supt6, SUPT6H
• Immunogen: Between 1050 and 1100
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG