Invitrogen™ Survivin Recombinant Mouse Monoclonal Antibody (STLALYV), Alexa Fluor™ Plus 647

Catalog No.	Quantity
74-005-5MP647	50 μL
50-003-32720	20 μL

Catalog No. 74-005-5MP647 Supplier Invitrogen™ Supplier No. 740055MP647

Description

Mouse Recombinant Monoclonal Antibody

Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro. Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 658 nm; Emission: 675 nm; Laser: Red Laser Filtration: 0.2 μm post-manufacturing filtered.

Survivin (IAP4) is the smallest member of the Inhibitors of Apoptosis Protein (IAP) gene family. The IAPs are involved in multiple cell functions such as cell signaling, cell division, metabolism, and exhibits differential expression in nearly all human cancers, but not in most normal tissues. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but the Survivin gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of survivin's gene expression. Survivin is expressed in the G2/M phase of the cell cycle in a cycle-regulated manner. At the beginning of mitosis, survivin associates with microtubules of the mitotic spindle in a specific and saturable reaction that is regulated by microtubule dynamics. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. Survivin may counteract a default induction of apoptosis in G2/M phase. Survivin is also abundantly expressed in brain tissues (astrocytes and some neurons) of adult rats following traumatic brain injury. Survivin has been found co-expressed with NeuN (mature neuronal marker) and PCNA (a cell cycle protein). Survivin might affect regulation of neural cell proliferative responses after brain injury. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined. The overexpression of survivin in cancer may overcome this apoptotic checkpoint and favor aberrant progression of transformed cells through mitosis.

Specifications

• Antigen: Survivin
• Applications: Flow Cytometry, Immunocytochemistry
• Classification: Recombinant Monoclonal
• Clone: STLALYV
• Concentration: 1 mg/mL
• Conjugate: Alexa Fluor Plus 647
• Formulation: Proprietary buffer with 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone; pH 6.3-7.3
• Gene: Birc5
• Gene Accession No.: O15392
• Gene Alias: AAC-11; AP14; Api4; apoptosis inhibitor 4; Apoptosis inhibitor survivin; baculoviral IAP repeat containing 5; baculoviral IAP repeat-containing 5; baculoviral IAP repeat-containing 5 (survivin); baculoviral IAP repeat-containing protein 5; BIRC 5; BIRC5; EPR 1; EPR-1; Iap4; inhibitor of apoptosis; survivin; survivin variant 3 alpha; survivin40; SVV; TIAP
• Gene Symbols: Birc5
• Host Species: Mouse
• Purification Method: Protein A/G
• Quantity: 50 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 332
• Target Species: Human
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2b κ