ATCC ATCC SW10 Schwann cell line (CRL-2766), mouse, adherent, temperature-sensitive

Supplier:  ATCC CRL2766

SW10 is a mouse Schwann cell line supplied by ATCC. The line is temperature-sensitive and can be cultured at a permissive temperature (33°C) for proliferation or at a restrictive temperature (37°C) for nonproliferative conditions. It is an adherent neuronal Schwann cell line suitable for in vitro studies of Schwann cell biology and nerve physiology.

• Species: Mouse (Mus musculus).
• Cell type: Neuronal Schwann cell line.
• Growth properties: Adherent.
• Recommended medium: DMEM supplemented with 10% fetal bovine serum.
• Culture conditions: 33°C permissive or 37°C restrictive, 5% CO2.
• Derivation: Primary Schwann cells transformed with a temperature-sensitive SV40 large T antigen.
• Subculture: Recommended split ratio 1:4 to 1:8.
• Cryopreservation: Store in vapor phase liquid nitrogen or below -130°C; freeze medium with 5% DMSO; do not store at -70°C.

Catalog No. NC3345115