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Sigma Aldrich Fine Chemicals Biosciences SYBRR Green Quantitative R
Supplier: Sigma Aldrich Fine Chemicals Biosciences QR01001KT
The SYBR(R) Green Quantitative RT-PCR Kit combines Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) JumpStart(TM) Taq DNA polymerase and SYBR Green I fluorescent dye in a one-step RT-PCR kit designed for measurement of gene expression. This convenient 2X ready mix includes M-MLV RT SYBR Green I dye JumpStart(TM) Taq DNA polymerase 99% pure deoxynucleotides buffer glass passivator and stabilizers. The JumpStart(TM) Taq DNA polymerase is an antibody-inactivated hot-start enzyme. Once the reaction temperature reaches 70C the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques.
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