Invitrogen™ Syrian Hamster IgG Polyclonal, Brilliant Ultra Violet™ 615, eBioscience™

Syrian Hamster Polyclonal Control

Supplier:  Invitrogen™ 366491481

Catalog No. 36-649-1481

Description

Description: This hamster IgG fraction was purified by affinity chromatography from normal Golden Syrian Hamster serum. Applications Reported: This antibody has been reported for use in flow cytometric analysis. Applications Tested: This isotype control antibody has been tested by flow cytometric analysis of mouse splenocytes. It should be used at the same concentration as the experimental antibody. Brilliant Ultra Violet™ 615 (BUV615) is a tandem dye that emits at 615 nm and is intended for use on cytometers equipped with an ultraviolet (355 nm) laser. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright, Brilliant Violet™ , Brilliant Ultra Violet™ , or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on br...

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Specifications

• Antigen: Syrian Hamster IgG
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!, 4°, C, store in dark, DO NOT FREEZE!
• Conjugate: Brilliant Ultraviolet 615
• Form: Liquid
• Host Species: Syrian Hamster
• Purification Method: Affinity chromatography
• Target Species: Not Applicable

• Applications: Control, Flow Cytometry
• Concentration: 0.2 mg/mL
• Formulation: PBS with BSA and 0.09% sodium azide, pH 7.2, PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Alias: IgG, Immunoglobulin G, ImmunoglobulinG
• Quantity: 50 μg
• Regulatory Status: RUO
• Isotype: IgG