Invitrogen™ Syrian Hamster IgG Isotype Control, PE-Cyanine5.5, eBioscience™

Catalog No.	Quantity
35-491-481	50 μg

Catalog No. 35-491-481 Supplier Invitrogen™ Supplier No. 35491481

Description

Syrian Hamster Polyclonal Isotype Control

Description: This hamster IgG fraction was purified by affinity chromatography from normal Golden Syrian hamster serum. Applications Reported: This isotype control antibody has been reported for use in flow cytometric analysis. Applications Tested: This isotype control antibody has been tested by flow cytometric analysis of mouse splenocytes. Use isotype control at the same concentration as the experimental antibody. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 695 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Specifications

• Antigen: Syrian Hamster IgG
• Applications: Control, Flow Cytometry
• Classification: Polyclonal
• Concentration: 0.2 mg/mL
• Conjugate: PE-Cyanine5.5
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: IgG; Immunoglobulin G; ImmunoglobulinG
• Host Species: Syrian Hamster
• Purification Method: Affinity chromatography
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Isotype Control (Primary)
• Target Species: Not Applicable
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Isotype Control
• Form: Liquid
• Isotype: IgG