Invitrogen™ Human TACI (TNFRSF13B) ELISA Kit

ELISA

Supplier:  Invitrogen™ EHTNFRSF13BX10

Catalog No. 50-255-0909

Description

The Human TACI (TNFRSF13B) ELISA quantitates Hu TACI in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TACI. Principle of the method The Human solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Members in the TNF superfamily regulate immune responses and induce apoptosis. Two novel members in the TNF family were recently identified and designated BAFF/BLyS/TALL-1/THANK/zTNF4 and April/TALL-2, respectively. BAFF was characterized as a B cell activator since it induced B cell proliferation and immunoglobulin secretion. April regulates immunological and non-immunological cell growth. Three receptors, BCMA, TACI, and BAFF-R, for BAFF and April were recently identified. TACI, like BCMA, binds BAFF and April. TACI and its ligands regulate humoral immune responses, activate NF-kappa-B and c-jun N-terminal kinase, and are involved in B cell associated autoimmune diseases.

Specifications

• Accession Number: O14836
• Assay Sensitivity: 0.3 ng/mL
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Sufficient For: 10 x 96 assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 23495
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 10 x 96 Tests
• Research Discipline: Immunology
• Storage Requirements: 2°C to 8°C
• Target: TACI
• Total Assay Time: 4 hrs 45 mins

• Assay Range: 0.323 to 80 ng/mL
• Calibration Range: 0.3 ng/mL to 80 ng/mL
• Conjugate: HRP
• Protein Family: Other Proteins
• Specificity: This ELISA pair antibody detects human TACI. Other species not determined.
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: CD267,CVID,CVID2,IGAD2,RYZN,TACI,TNFRSF14B
• Intraassay CV: <10
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Synonym: CD267, CVID, CVID2, IGAD2, RYZN, TACI, TNFRSF14B
• Test Time: 1 hrs 20 mins