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Thermo Scientific™ Tango™ H4-bla U2OS

Catalog No. K1465
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8 x 108 Cells
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K1465 8 x 108 Cells
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Catalog No. K1465 Supplier Thermo Scientific™ Supplier No. K1465
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Description

The Tango™ H4-bla U2OS cells contain the human Histamine Receptor 4 (H4) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line.

The Tango™ H4-bla U2OS cells contain the human Histamine Receptor 4 (H4) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ H4-bla U2OS cells are functionally validated for Z' and EC50 concentrations of 4-Methylhistamine. In addition, Tango™ H4-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.

TRUSTED_SUSTAINABILITY

Specifications

Accession Number NP_067637.2
Assay Entry Cell-based beta-arrestin recruitment
Cell State Division-Arrested Cells
Content And Storage LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.

Includes:
• Tango™ H4-bla U2OS cells
• Tango™ H4-bla U2OS Assay Protocol
• Certificate of Analysis

The Tango™ H4-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Cytoplasmic Tail Tail from AVPR2 GPCR
Detection Method Fluorescence
For Use With (Application) FRET
Gene Symbol HRH4
Population Status Single Cell Clone Origin
Product Line GeneBLAzer, Tango
Quantity 8 x 108 Cells
Readout End Point
Reporter Gene BLA (Beta-Lactamase)
Target Entry HRH4, H4
Cell Line U2OS
System Type Tango™
Druggable Target GPCR
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For Research Use Only. Not for use in diagnostic procedures.

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