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Thermo Scientific™ Tango™ PTAFR-bla U2OS Cells

Catalog No. K1789
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8 x 106 Cells
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K1789 8 x 106 Cells
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Catalog No. K1789 Supplier Thermo Scientific™ Supplier No. K1789
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Description

The Tango™ PTAFR-bla U2OS cells contain the human Platelet Activating Factor Receptor (PTAFR) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line.

The Tango™ PTAFR-bla U2OS cells contain the human Platelet Activating Factor Receptor (PTAFR) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ PTAFR-bla U2OS cells are functionally validated for Z' and EC50 concentrations of β-Acetyl-γ-O-hexadecyl-L-α-phosphatidylcholine. In addition, Tango™ PTAFR-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.

TRUSTED_SUSTAINABILITY

Specifications

Accession Number NM_000952
Assay Entry Cell-based beta-arrestin recruitment
Cell State Dividing Cells
Content And Storage LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.

Includes:
• Tango™ PTAFR-bla U2OS cells
• Tango™ PTAFR-bla U2OS Assay Protocol
• Certificate of Analysis

The Tango™ PTAFR-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Cytoplasmic Tail Native Tail
Detection Method Fluorescence
For Use With (Application) FRET
Gene Symbol PTAFR
Population Status Pool of Stable Cells
Product Line GeneBLAzer
Quantity 8 x 106 Cells
Readout End Point
Reporter Gene BLA (Beta-Lactamase)
Target Entry PTAFR
Cell Line U2OS
System Type Tango™
Druggable Target GPCR
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For Research Use Only. Not for use in diagnostic procedures.

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