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Applied Biosystems™ TaqMan™ Copy Number Reference Assay, human, RNase P

Human TaqMan™ Copy Number Reference Assays are run with human TaqMan™ Copy Number Assays in a duplex real-time PCR reaction to detect and measure copy number variations (CNVs) and smaller regions in the human genome.

Manufacturer:  Applied Biosystems™ 4403326

Encompass

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Catalog No. 44-033-26


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Description

Description

  • Assays are run with human TaqMan™ Copy Number Assays in a duplex real-time PCR reaction
  • Used to detect and measure copy number variations (CNVs) and smaller regions in the human genome
  • Two options for genes that can be used as endogenous reference genes in humans are offered: RNase P and TERT
  • Designed to unique genomic sequences in the reference genome assembly (build GRCh38) and are required for relative quantitation of copy number targets
  • TaqMan™ Copy Number Assays quantitate the gene of interest and normalize to an endogenous reference gene known to be present in two copies in a diploid genome
  • Please note that TaqMan™ Copy Number Reference Assays are species specific.
  • Assays should be stored at -15°C to -25°C in the dark
  • Minimize freeze-thaw cycles

TaqMan™ Copy Number Reference Assay RNase P

  • Recommended as the standard reference assay for copy number analysis
  • This assay detects the Ribonuclease P RNA component H1 (H1RNA) gene (RPPH1) on chromosome 14, cytoband 14q11.2
  • The assay location is chr.14:20343370 on build GRCh38
  • It has an 87 bp amplicon that maps within the single exon RPPH1 gene

TaqMan™ Copy Number Reference Assay TERT

  • An alternative reference assay recommended in the event that the RNase P assay functions poorly with a sample
  • This could be due to chromosomal aberrations or other issues
  • This assay targets the telomerase reverse transcriptase (TERT) gene located on chromosome 5, cytoband 5p15.33
  • The assay location is chr5:1253257 on build GRCh38
  • It has an 88 bp amplicon that maps within exon 16 of the TERT gene
Specifications

Specifications

None
Contains one tube (750 μL) of 20X pre-formulated assay with reference sequence-specific forward and reverse primers and 1 VIC™ dye-labeled TAMRA™ probe. The tube contains enough assay for 750 20-μl reactions or 1500 10-μl reactions in 96-well format plates. Assays should be stored at -15°C to -25°C in the dark. Minimize freeze-thaw cycles.
Primer-Probe
Tube
750 Reactions
Standard
Sustainable packaging
TAMRA™ Quencher (3'), VIC™ (5')
TaqMan™
HPLC
Ambient Temperature
750 Reactions
None
20X
Frozen
Low
qPCR
RPPH1
Control Primer-Probe Set(s)
VIC
Configurable Assay
RNase-Free, DNase-Free
Human
RNase P
SDS
Documents

Documents

Product Certifications
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