Applied Biosystems™ TaqMan™ GAPDH Control Reagents (human)

Catalog No.	Quantity
40-286-9	100 reactions

Catalog No. 40-286-9 Supplier Applied Biosystems™ Supplier No. 402869

Description

TaqMan™ GAPDH Control Reagents (human)

The Applied Biosystems™ TaqMan™ GAPDH Control Reagents kit provides components for using human GAPDH as a normalization control in your real-time PCR reactions. The kit includes human control RNA, GAPDH TaqMan™ probe (JOE™ dye-labeled probe), and GAPDH primers. You can use either DNA or RNA as a target template.

Pre-designed primers and JOE™ dye-labeled TaqMan™ probe eliminate assay design

Rapid assay development guidelines can minimize optimization time

Separate components provide flexibility in assay set-up

Comprehensive Guidelines Included
Applied Biosystems™ has developed a comprehensive set of guidelines to ensure success when using Applied Biosystems™ Real-Time PCR reagents and instrumentation. These guidelines are simple and easy to follow. Many traditional variables, such as magnesium chloride concentration and the thermal cycling protocol itself, have been standardized, greatly reducing assay development time.

Normalization Built-In
All Applied Biosystems™ Real-Time PCR reagent kits contain a passive internal reference to normalize non-PCR related fluorescence fluctuations. Normalizing with a passive internal reference minimizes well-to-well variability that can result from a variety of causes, such as pipetting errors and sample evaporation. The passive reference is also essential for accurate results when multiple probes (with different reporter dyes) are combined in a single tube.

Notes on Use
The TaqMan™ GAPDH Control Reagents can be used with TaqMan™ Universal PCR Master Mix or TaqMan™ PCR Core Reagents for polymerase chain reaction (PCR).The TaqMan™ GAPDH Control Reagents can be used with the TaqMan™ One-Step RT-PCR Master Mix Reagents, TaqMan™ EZ RT-PCR Core Reagents, or the TaqMan™ Gold RT-PCR Reagents for one-step reverse transcription polymerase chain reaction (RT-PCR).

The PCR process and 5' nuclease process are covered by patents owned by Roche Molecular Systems, Inc. and F. Hoffmann-La Roche, Ltd.

Note: See user's manual or package insert for limited label license, and trademark information. For Research Use Only. Not for use in diagnostics procedures.

Specifications

• Content And Storage: Control Primer-Probe Set(s) & Template(s)
• Control Template: Human Control RNA
• Format: Tube
• GC-Rich PCR Performance: Low
• PCR Method: 1-step RT-qPCR
• Reaction Speed: Standard
• Gene Symbol: GADPH
• Internal Probe Modification: TAMRA™ Quencher (3'), JOE (5')
• Label or Dye: JOE
• Product Line: TaqMan
• Product Type: Real Time PCR Reagents
• Quantity: 100 reactions
• Shipping Condition: Dry Ice
• Species: Human
• Sufficient For: 100 Reactions
• Target: GAPDH
• No. of Reactions: 100 Reactions

Frequently Asked Questions (FAQs)

In the TaqMan GAPDH Control Reagents Kit (Cat. No. 402869), what is the size of the GAPDH amplicon?

The amplicon length is 226 bases, and is based on the NCBI GenBank accession number J04038. More information on the GAPDH amplicon can be found in Appendix B of the “TaqMan Gold RT-PCR Kit” protocol that is found on the Product Detail Page for Cat. No. 402869. Enter the catalog number in the main Search field on our web site to view this page and the Protocol.

Do the human and rodent GAPDH primers and TaqMan probe sequences manufactured by Applied Biosystems detect genomic DNA?

This depends on the individual probe. Potential reaction with genomic DNA - based on position of primers and/or probe within or across exon junctions - is one of the specifications indicated in the Assay search tool and product support materials. Here are details on the reported performance of a few specific products:

- TaqMan GAPDH Control Reagents, human, 402869, JOE: Does not detect genomic DNA
- TaqMan Endogenous Control, GAPDH for human, 4326317E, VIC : Does not detect genomic DNA
- TaqMan Endogenous Control, GAPDH, human, 4310884E, VIC: Does not detect genomic DNA
- TaqMan Endogenous Control, GAPDH, human, 4333764F, FAM: Does not detect genomic DNA
- TaqMan Endogenous Control, GAPDH, human, 4333764T, FAM: Does not detect genomic DNA
- TaqMan Gene Expression Assay, GAPDH, human, Hs99999905_m1/4331182, FAM: Does not detect genomic DNA

Do your human and rodent GAPDH primers and TaqMan probe sequences detect genomic DNA?

It varies by product, so be sure to check all the available product specifications before purchase. Here are details on a few options:

- The TaqMan GAPDH Control Reagents (Human), Cat. No. 402869, which is labeled with JOE dye, spans two exons therefore will not amplify genomic DNA.

- The Human GAPD (GAPDH) Endogenous Control (VIC/ TAMRA Probe, Primer Limited), Cat. No. 4310884E, is also designed across an exon-exon boundary and will not amplify genomic DNA.

- The probe in the TaqMan Rodent GAPDH Control Reagents kit, Cat. No. 4308313, labeled with VIC dye, does not span an intron and therefore may amplify genomic DNA.

For Research Use Only. Not for use in diagnostic procedures.