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Applied Biosystems™ TaqMan™ hPSC Scorecard™ Kit, 384-well Non-distribution product as customer accommodation.

TaqMan™ hPSC Scorecard™ Kit, 384-well

Manufacturer:  Applied Biosystems™ A15872

Catalog No. A15872


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Description

Description

The Applied Biosystems™ TaqMan™ hPSC Scorecard™ Kit 384w enables verification of trilineage differentiation potential for both human ES and iPS cell lines. The 384-well plate contains four sets of 94 predefined TaqMan™ Gene Expression assays (including endogenous controls) dried-down in the wells. Master mix is included for your convenience. To purchase the panel alone, see TaqMan™ hPSC Scorecard™ Panel 384w. This product is also available in a 2 x 96w Fast format.

  • Assess the gene signature of four samples in one simple experiment
  • Compare results to a reference standard with the accompanying hPSC Scorecard™ Analysis Software
  • Identify lines with trilineage differentiation potential
  • Evaluate inductive differentiation specified to one germ layer
  • Access validated content for increased confidence in your results

    Assess an Entire Gene Signature in One Experiment
    The TaqMan™ hPSC Scorecard™ Panel helps save time by offering pre-plated assays in a stable and convenient dried-down format. Simply add TaqMan™ Master Mix to your cDNA of interest and transfer to the 384-well plate with a multichannel pipette.

    Compare Results to a Reference Standard
    The TaqMan™ hPSC Scorecard™ Panel includes access to proprietary cloud-based analysis software that allows you to view and export graphs and tables of results, as well as generate reports. The software is compatible with seven Applied Biosystems™ qRT-PCR systems and is available at no additional charge.

    Identify Lines with Trilineage Differentiation Potential
    Include randomly differentiated embryoid body (EB) samples (differentiated for at least 7 days using standard EB methods) in your analysis and determine whether your lines are biased toward one or more of the three germ layers (endoderm, mesoderm, ectoderm).

    Evaluate Inductive Differentiation Specified to One Germ Layer
    The panel includes over twenty markers for each of the three germ layers that have been shown to respond as expected to directed differentiation in monolayer culture. Quickly evaluate time course data, culture conditions and media formulations for your germ layer of interest with this one panel.

    Access Validated Content
    The panel contents are based on published work (Bock et al., Cell 144, 439–452, 2011) and were validated against multiple human ES and iPS lines.

    What You Get
    The TaqMan™ hPSC Scorecard™ Panel 384w includes:

  • One 384-well plate of 94 TaqMan™ Gene Expression assays (4 gene sets per plate)
  • One optical plate cover
  • One 5-mL vial of TaqMan™ Gene Expression Master Mix
  • Specifications

    Specifications

    TaqMan™ hPSC Scorecard™ Kit, 384 rxns, Dried Down, 1 x 384 Well Plate, Human Species, Standard Reaction Speed, 2 to 8°C Recommended Storage, For Use with QuantStudio™ 12K Flex, ViiA™ 7 System, For Stem Cell Analysis
    Primer-probe
    One 384-well plate with four replicates of the 96-assay panel (room temperature), One optical cover (room temperature), One 5mL vial of TaqMan™ Gene Expression Master Mix
    384 rxn(s)
    Stem cell analysis
    QuantStudio™ 12K Flex, ViiA™ 7 System
    Safety and Handling

    Safety and Handling

    Recommended Storage : 2 to 8°C

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    For Research Use Only. Not for use in diagnostic procedures.