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TaqMan™ miRNA ABC Purification Kit - Human Panel A
Specifications
Specifications
| Content And Storage | Buffer Kit - Store at room temperature Lysis Buffer ABC Buffer Wash Buffer 1 Wash Buffer 2 Elution Buffer 1.5 mL LoBind tubes Human Panel A Beads Store at 2 °C to 8 °C |
| Format | Kit |
| Isolation Technology | Magnetic Bead |
| Purification Time | 75 min. |
| RNAi Type | miRNA |
| Species | Human |
| Sample Type | Cell Cultures, Plasma, Saliva, Serum, Tissue, Tissue (FFPE), Tissue Sections, Urine, Whole Blood |
| Final Product Type | micro RNA |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| For Use With (Application) | Real-Time Quantitative PCR (qPCR), microRNA Analysis |
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Frequently Asked Questions (FAQs)
Here are possible causes and solutions:
- RNA degradation due to RNAse contamination during procedure: See “Guidelines to prevent RNAse contamination” on page 12 of the manual (https://tools.thermofisher.com/content/sfs/manuals/4473439C.pdf) for proper handling of sample.
- Inefficient elution of miRNA from the beads: follow hybridization, wash, and elution steps on pages 17-18.
- Inefficient washing: fully resuspend beads in wash buffers, and completely remove buffers at the end of the washes.
- Beads dried out during procedure: prepare the beads just before use and keep the beads suspended in liquid and the tube cap closed in between steps.
- Buffers not diluted as directed: prepare buffers as directed on page 13.
- Kit components compromised due to inappropriate storage conditions: store reagents as directed in “Kit contents and storage conditions” on page 9 of the manual.
Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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