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Applied Biosystems™ TaqMan™ Mutation Detection Assay with castPCR™ Technology
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Catalog No. 4465804
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Gene Reference (75 reactions), Inventoried
Mutant Allele (75 reactions), Inventoried
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Catalog No. Quantity
44-658-04 Mutant Allele (75 reactions), Inventoried
44-658-07 Gene Reference (75 reactions), Inventoried
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Catalog No. 44-658-04 Supplier Applied Biosystems™ Supplier No. 4465804
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Description

Powered by competitive allele-specific TaqMan™ PCR (castPCR™ Technology) to detect and measure somatic mutations in genes associated with cancer research.

The castPCR technology is highly specific and sensitive, and can detect rare amounts of mutated DNA in a sample that contains large amounts of normal, wild-type DNA. These assays are compatible with different sample types, such as cell lines, formalin-fixed paraffin-embedded (FFPE) tissue samples, and fresh frozen tissue samples.

Competitive, allele-specific TaqMan PCR utilizes an allele-specific primer for mutant allele detection that competes with an MGB blocker oligonucleotide to suppress the wild-type background.

  • Higher specificity: designed to block the wild-type and amplify only the mutant
  • Higher sensitivity: designed to detect somatic mutations down to 1 cancer cell in 1000 normal cells
  • Faster workflow: 3 hours from sample to result
  • Detect multiple types of mutations: detect single- and multiple-nucleotide mutations and insertion/deletions (indels)
  • Wide dynamic range and excellent PCR efficiency: assays demonstrate at least 4 logs of dynamic range and an average efficiency of 100% ± 10%
  • Fast, simple workflow: like other TaqMan Assays, typically require 3 hours from sample to results, with minimum hands-on time

Order Info

Expiration date 2 years from the date of manufacturer. Shipping Conditions: Ambient Temperature

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Specifications

Concentration 10X
Content And Storage 1 tube containing a 10X mix of unlabeled PCR primers, a TaqMan MGB probe, and an MGB oligonucleotide blocker.

Store at -15 to -25°C.
Detection Method Primer-probe
Form Frozen
Format Tube
GC-Rich PCR Performance High
PCR Method qPCR
Reaction Speed Fast
Product Line TaqMan
Product Type Mutation Detection Assay
Quantity Mutant Allele (75 reactions), Inventoried
Shipping Condition Room Temperature
Fidelity (vs. Taq) 10 X
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