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Applied Biosystems™ TaqMan™ RNase P Detection Reagents Kit

TaqMan™ RNase P Detection Reagents Kit

Manufacturer:  Applied Biosystems™ 4316831

Encompass

Catalog No. 43-168-31


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Description

Description

The Applied Biosystems™ TaqMan™ RNase P Detection Reagents kit provides the components needed to detect and quantitate genomic copies of the human RNase P gene using the 5' nuclease assay. Human RNase P gene is a single-copy gene encoding the RNA moiety for the RNase P enzyme. This 5' nuclease assay employs TaqMan™ Universal PCR Master Mix or TaqMan™ PCR Core Reagents for polymerase chain reaction (PCR) using the provided human genomic DNA as template.

  • Pre-designed primers and TaqMan™ probe eliminate assay design
  • Easy-to-use primer and TaqMan™ probe mix minimizes assay set-up time
  • Rapid Assay Development Guidelines can minimize optimization time

    Comprehensive Guidelines Included
    Applied Biosystems™ has developed a comprehensive set of guidelines to ensure success when using Applied Biosystems™ Real-Time PCR reagents and instrumentation. These guidelines are simple and easy to follow. Many traditional variables, such as magnesium chloride concentration and the thermal cycling protocol itself, have been standardized, greatly reducing assay development time.

    Normalization Built-In
    All Applied Biosystems™ Real-Time PCR reagent kits contain a passive internal reference to normalize non-PCR related fluorescence fluctuations. Normalizing with a passive internal reference minimizes well-to-well variability that can result from a variety of causes, such as pipetting errors and sample evaporation. The passive reference is also essential for accurate results when multiple probes (with different reporter dyes) are combined in a single tube.

    Note: See user's manual or package insert for limited label license, and trademark information. For Research Use Only. Not for use in diagnostics procedures.
  • Specifications

    Specifications

    Passive Internal Reference Standards
    Human Genomic DNA
    Low
    qPCR
    Control Primer-Probe Set(s) & Template(s)
    FAM
    Real Time PCR Reagents
    Human
    RNase P
    Includes: 20X Primer and TaqMan™ Probe (FAM™ probe, with TAMRA™ quencher) mix and Human Genomic DNA.

    Storage: -20°C
    Tube
    100 Reactions
    Standard
    TAMRA™ Quencher (3'), FAM (5')
    TaqMan™
    Dry Ice
    100 Reactions
    SDS
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    For Research Use Only. Not for use in diagnostic procedures.