Applied Biosystems™ TaqMan™ Rodent GAPDH Control Reagents

Catalog No.	Quantity
43-083-13	1,000 reactions

Catalog No. 43-083-13 Supplier Applied Biosystems™ Supplier No. 4308313

Description

TaqMan™ Rodent GAPDH Control Reagents

The Applied Biosystems™ TaqMan™ Rodent GAPDH Control Reagents provide the components for using rodent GAPDH as a normalization control in applications with rat, mouse, and Chinese hamster cells.

Pre-designed primers and TaqMan™ probe eliminate assay design

Rapid assay development guidelines can minimize optimization time

Separate components provide flexibility in assay set-up

Comprehensive Guidelines Included
Applied Biosystems™ has developed a comprehensive set of guidelines to ensure success when using Applied Biosystems™ Real-Time PCR reagents and instrumentation. These guidelines are simple and easy to follow. Many traditional variables, such as magnesium chloride concentration and the thermal cycling protocol itself, have been standardized, greatly reducing assay development time.

Normalization Built-In
All Applied Biosystems™ Real-Time PCR reagent kits contain a passive internal reference to normalize non-PCR related fluorescence fluctuations. Normalizing with a passive internal reference minimizes well-to-well variability that can result from a variety of causes, such as pipetting errors and sample evaporation. The passive reference is also essential for accurate results when multiple probes (with different reporter dyes) are combined in a single tube.

Notes on Use:
The TaqMan™ Rodent GAPDH Control Reagents can be used with TaqMan™ Universal PCR Master Mix or TaqMan™ PCR Core Reagents for polymerase chain reaction (PCR). The TaqMan™ Rodent GAPDH Control Reagents can be used with TaqMan™ One-Step RT-PCR Master Mix Reagents, TaqMan™ EZ RT-PCR Core Reagents, or the TaqMan™ Gold RT-PCR Reagents for one-step reverse transcription polymerase chain reaction (RT-PCR).

Note:
See user's manual or package insert for limited label license, and trademark information. For Research Use Only. Not for use in diagnostics procedures.

Specifications

• Content And Storage: Control Primer-Probe Set(s) & Template(s)
• Control Template: Control Mouse RNA
• Format: Tube
• GC-Rich PCR Performance: Low
• PCR Method: 1-step RT-qPCR
• Reaction Speed: Standard
• Gene Symbol: GAPDH
• Internal Probe Modification: TAMRA™ Quencher (3'), VIC™ (5')
• Label or Dye: VIC
• Product Line: TaqMan
• Product Type: Real Time PCR Reagents
• Quantity: 1,000 reactions
• Shipping Condition: Dry Ice
• Species: Mouse, Hamster, Rat
• Sufficient For: 1000 Reactions
• Target: GAPDH
• No. of Reactions: 1000 Reactions

Frequently Asked Questions (FAQs)

What type of control RNA is included in the TaqMan Rodent GAPDH kit (P/N 4308313)?

The RNA included in the TaqMan Rodent GAPDH kit is total RNA extracted from P388D1 lymphoma mouse cell line.

What is the GenBank accession number of the sequence that was used to design the primers and probe for the TaqMan Rodent GAPDH Control Reagent Kit (P/N 4308313)?

The GenBank accession number used is NM_008084. For more information on this kit, please refer to the TaqMan Rodent GAPDH kit protocol.

Since the TaqMan Rodent GAPDH Control Reagents (Cat. No. 4308313) will also amplify pseudogenes and/or genomic DNA, is there some equation that can be used to correct for genomic DNA contamination?

There is no equation for the correction of data for genomic contamination; however, a rule of thumb is that if the Ct difference between your target and the contaminant is 10 or more, you can assume it is not having an effect in your reaction. Please consult the TaqMan Rodent GAPDH Control Reagents Protocol for more information on these reagents. Enter the catalog number in the main Search field on our web site to view the product detail page and the Protocol.

Do the human and rodent GAPDH primers and TaqMan probe sequences manufactured by Applied Biosystems detect genomic DNA?

This depends on the individual probe. Potential reaction with genomic DNA - based on position of primers and/or probe within or across exon junctions - is one of the specifications indicated in the Assay search tool and product support materials. Here are details on the reported performance of a few specific products:

- TaqMan GAPDH Control Reagents, human, 402869, JOE: Does not detect genomic DNA
- TaqMan Endogenous Control, GAPDH for human, 4326317E, VIC : Does not detect genomic DNA
- TaqMan Endogenous Control, GAPDH, human, 4310884E, VIC: Does not detect genomic DNA
- TaqMan Endogenous Control, GAPDH, human, 4333764F, FAM: Does not detect genomic DNA
- TaqMan Endogenous Control, GAPDH, human, 4333764T, FAM: Does not detect genomic DNA
- TaqMan Gene Expression Assay, GAPDH, human, Hs99999905_m1/4331182, FAM: Does not detect genomic DNA

In the TaqMan Rodent GAPDH Control Reagents kit (P/N 4308313), what rodent is the Control RNA isolated from?

The control RNA is total RNA isolated from a mouse lymphoma cell line.

Are there any TaqMan Endogenous Controls reagents available that can be used with rat samples?

We have TaqMan Endogenous Controls for 18S Ribosomal RNA and a kit (Cat. No. 4308313) that can be used with rat samples. The Eukaryotic 18S Ribosomal RNA Reagent is in a highly conserved area that allows this TaqMan Endogenous Control to be used for rat, human and mouse. In addition, we have newer TaqMan Gene Expression Rat Endogenous Controls, including beta glucuronidase and beta-2 microglobulin. These can be found by searching our Gene Expression Assays and specifying the species of interest as R. norvegicus and the set membership as Endogenous Controls.

For Research Use Only. Not for use in diagnostic procedures.