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MilliporeSigma™ TAT-CRE Recombinase Protein

Catalog No. SCR508 Shop All MilliporeSigma Products
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1500 U
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SCR508 1500 U
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Catalog No. SCR508 Supplier MilliporeSigma™ Supplier No. SCR508
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Description

Catalyzes site specific recombination event between two loxP DNA sites. MilliporeSigma™; TAT-CRE Recombinase is a recombinant cell-permeant fusion cre-recombinase protein consisting of TAT sequence, a nuclear localization sequence (NLS).

  • Consists of basic protein translocation peptide derived from HIV-TAT (TAT), a nuclear localization sequence (NLS), the Cre protein and an N-terminal histidine tag (H6) for efficient purification of the protein from E. coli
  • Effectively excises STEMCCA viral transgenes from both Human and Mouse IPS cells
  • Purity: single band around 41kDa with greater than 70% protein purity on SDS-PAGE gel
  • Functional activity: Mediates recombination of LoxP-modified alleles in HEK293T- Cre reporter cell line 
  • Endotoxin levels: less than 1 EU/ug protein
  • Mycoplasma negative
TRUSTED_SUSTAINABILITY

Specifications

For Use With (Application) Cell Culture, Stem Cell Culture
Quantity 1500 U
Source E.coli
Storage Requirements Stable for 3 months from date of receipt when stored at -20°C or -80°C. Upon first thaw, centrifuge the vial and gently mix the solution. Aliquot into smaller working volumes and freeze at -20°C or -80°C.
Endotoxin Concentration <1 EU/ug protein
Product Type TAT-CRE Recombinase Protein
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