MilliporeSigma™ TAT-CRE Recombinase Protein

Description

Catalyzes site specific recombination event between two loxP DNA sites. MilliporeSigma™; TAT-CRE Recombinase is a recombinant cell-permeant fusion cre-recombinase protein consisting of TAT sequence, a nuclear localization sequence (NLS).

• Consists of basic protein translocation peptide derived from HIV-TAT (TAT), a nuclear localization sequence (NLS), the Cre protein and an N-terminal histidine tag (H6) for efficient purification of the protein from E. coli
• Effectively excises STEMCCA viral transgenes from both Human and Mouse IPS cells
• Purity: single band around 41kDa with greater than 70% protein purity on SDS-PAGE gel
• Functional activity: Mediates recombination of LoxP-modified alleles in HEK293T- Cre reporter cell line 
• Endotoxin levels: less than 1 EU/ug protein
• Mycoplasma negative

Specifications

• For Use With (Application): Cell Culture, Stem Cell Culture
• Quantity: 1500 U
• Source: E.coli
• Storage Requirements: Stable for 3 months from date of receipt when stored at -20°C or -80°C. Upon first thaw, centrifuge the vial and gently mix the solution. Aliquot into smaller working volumes and freeze at -20°C or -80°C.
• Endotoxin Concentration: <1 EU/ug protein
• Product Type: TAT-CRE Recombinase Protein