Invitrogen™ TCP1 Monoclonal Antibody (91A)

Rat Monoclonal Antibody

Supplier:  Invitrogen™ MA3026

Catalog No. MA3026

Description

MA3-026 detects T Complex Polypeptide 1 (TCP-1) from human, canine, chicken, hamster, mouse, primate, rat and yeast samples. MA3-026 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this clone detects a ~57 kDa band representing TCP-1 in mouse 3T3 cells. Immunofluorescence staining of TCP-1 in mouse germ line cells with MA3-026 results in diffuse cytoplasmic staining. The MA3-026 immunizing peptide corresponds to amino acid residues 306-556 from mouse TCP-1. The epitope for this antibody has been mapped to amino acids 465-469 representing the pentamer peptide AKLRA.

The T Complex Polypeptide 1 (TCP-1) is approximately 60 kDa protein constitutively expressed in almost all eukaryotic cells, and is upregulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60, Escherichia coli GroEL, the plastid Rubisco-subunit binding protein, and the archaebacterial protein TF55. The TCP-1 sequence shows nearly 40% identity to TF55, but only minimal similarity to HSP60 and GroEL.

Specifications

• Antigen: TCP1
• Classification: Monoclonal
• Concentration: Conc. Not Determined
• Formulation: ascites with 0.05% sodium azide
• Gene Accession No.: P11983, P12612, P17987, P18279, P28480
• Gene Symbols: TCP1
• Immunogen: Bacterially expressed fragment of mouse TCP-1, residues 306-556.
• Regulatory Status: RUO
• Gene ID (Entrez): 100689476, 21454, 24818, 421586, 484064, 6950, 851798
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: 91A
• Conjugate: Unconjugated
• Gene: TCP1
• Gene Alias: 65 kDa antigen; AI528772; c-cpn; CCT; Cct1; Ccta; CCTalpha; CCT-alpha; chaperonin-containing T-complex alpha subunit TCP1; chaperonin-containing T-complex polypeptide alpha subunit; cytosolic chaperonin containing t-complex polypeptide 1; D6S230E; hypothetical protein; p63; tailless complex polypeptide 1; tailless complex polypeptide 1A; Tailless complex polypeptide 1B; t-complex 1; t-complex polypeptide 1; t-complex protein 1; T-complex protein 1 alpha subunit-like protein; T-complex protein 1 subunit alpha; T-complex protein 1 subunit alpha A; T-complex protein 1 subunit alpha B; T-complex protein 1, alpha subunit; Tcp1; Tcp-1; TCP-1-A; TCP-1-alpha; TCP-1-B; Tp63; TRic; Unknown (protein for MGC:133746); YD8142.13; YD8142B.04; YDR212W
• Host Species: Rat
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Canine, Monkey, Hamster, Chicken, Yeast
• Product Type: Antibody
• Isotype: IgG2a

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov