Invitrogen™ TCR gamma/delta Monoclonal Antibody (B1.1), Functional Grade, eBioscience™, Invitrogen™

Catalog No.	Quantity
16-995-981	50 μg

Catalog No. 16-995-981 Supplier Invitrogen™ Supplier No. 16995981

Description

Mouse Monoclonal Antibody

The B1.1 MAb reacts with the human gamma delta TCR complex. The gamma delta TCR is expressed by a small subset of T cells in the thymus, peripheral lymphoid tissues, intestinal epithelium, and epidermis. The exact specificity, ligand and function of gamma delta TCR-bearing T cells are not yet fully understood; it is suggested that these cells recognize bacterial ligands and some tumor cells in the context of MHC class I-like gene products and play a role in regulation of the immune response and during bacterial infection. This monoclonal can be used as a phenotypic marker for gamma delta TCR-expressing T cells. Applications Reported: This B1.1 antibody has been reported for use in flow cytometric analysis. Applications Tested: The B1.1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/ μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

TCR gamma/delta (T-cell receptor gamma/delta) are specialized T-cells in the immune system. The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory auto-destructive reactions and are believed to be a method by which the immune system compensates for disease. Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). However, a small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.

Specifications

• Antigen: TCR gamma/delta
• Applications: Flow Cytometry, Functional Assay
• Classification: Monoclonal
• Clone: B1.1
• Concentration: 1 mg/mL
• Conjugate: Functional Grade
• Formulation: PBS with no preservative; pH 7.2
• Gene: TRD
• Gene Accession No.: 0
• Gene Alias: RGD1311300; similar to T cell receptor V delta 6; T cell receptor gamma locus; T3/TCR complex; TCR delta gamma; TCR gamma/delta; TCR-CD3 complex; Tcrg; Trg; Trg@
• Gene Symbols: TRD, Trg
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 6964, 6965
• Target Species: Human
• Content And Storage: 4°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1 κ

Safety and Handling

WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov