Invitrogen™ TCR gamma/delta Monoclonal Antibody (V65), FITC, eBioscience™

Catalog No.	Quantity
50-986-4	50 μg

Catalog No. 50-986-4 Supplier Invitrogen™ Supplier No. 11581081

Description

Mouse Monoclonal Antibody

Description: The V65 monoclonal antibody reacts with the gamma/delta chain of rat TCR. gammadelta TCR is expressed by dendritic epidermal T cells. Cross-linking of the TCR complex with V65 induces cytokine-dependent T cell stimulation. Applications Reported: The V65 antibody has been reported for use in flow cytometric analysis. Applications Tested: The V65 antibody has been tested by flow cytometric analysis of rat splenocytes and lymph node cells. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

TCR gamma/delta (T-cell receptor gamma/delta) are specialized T-cells in the immune system. The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory auto-destructive reactions and are believed to be a method by which the immune system compensates for disease. Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). However, a small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.

Specifications

• Antigen: TCR gamma/delta
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: V65
• Concentration: 0.5 mg/mL
• Conjugate: FITC
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene: Trg
• Gene Accession No.: 0
• Gene Alias: RGD1311300; similar to T cell receptor V delta 6; T cell receptor gamma locus; T3/TCR complex; TCR delta gamma; TCR gamma/delta; TCR-CD3 complex; Tcrg; Trg; Trg@
• Gene Symbols: Trg
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 24821
• Target Species: Rat
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov