PeproTech TECK Polyclonal Antibody, PeproTech®, Invitrogen™

Catalog No.	Quantity
500P134100	100 μg
500P13450UG	50 μg
500P1341MG	1 mg

Catalog No. 500P134100 Supplier PeproTech Supplier No. 500P134100UG

Description

Rabbit Polyclonal Antibody

AA Sequence of recombinant protein: QGVFEDCCLA YHYPIGWAVL RRAWTYRIQE VSGSCNLPAA IFYLPKRHRK VCGNPKSREV QRAMKLLDAR NKVFAKLHHN MQTFQAGPHA VKKLSSGNSK LSSSKFSNPI SSSKRNVSLL ISANSGL. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human TECK (CCL25). Anti-Human TECK (CCL25)-specific antibody was purified by affinity chromatography employing an immobilized Human TECK (CCL25) matrix. Sandwich ELISA: To detect Human TECK (CCL25) by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human TECK (CCL25) (500-P134Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human TECK (CCL15). Western Blot: To detect Human TECK (CCL25) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human TECK (CCL25) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Chemokines are likely to play an important role in regulating the trafficking of developing T cells within the thymus. Chemokine C-C thymus expressed chemokine (TECK), also designated chemokine ligand 25 (CCL25), small induc-ible cytokine A25, chemokine b-15 or CK b-15, is expressed predominantly in thymic dendritic cells, thymic epithelial cells and in the small intestine. TECK, a CCR9 ligand, has suppressive activity against immature subsets of myeloid progenitors which have been stimulated to proliferate by multiple growth factors. TECK delivers signals through CCR9, which is important for the nav-igation of developing thymocytes. Bone marrow pre-pro-B cells and cells capable of generating pro-B colonies in the presence of interleukin-7 and Flt-3 ligand migrate to TECK, a response lost in later stages of B cell development.

Specifications

• Antigen: TECK
• Applications: ELISA, Western Blot
• Classification: Polyclonal
• Concentration: 0.1-1.0 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with no preservative
• Gene: CCL25
• Gene Accession No.: O15444
• Gene Alias: A130072A22Rik; AI852536; C-C motif chemokine 25; C-C motif chemokine ligand 25; CCL25; chemokine (C-C motif) ligand 25; chemokine TECK; Ck beta-15; CKb15; Scya25; SCYA25TECKvar; small inducible chemokine 25; small inducible cytokine A25; small inducible cytokine subfamily A (Cys-Cys), member 25; small-inducible cytokine A25; Teck; TECKMGC150327; TECKvar; thymus expressed chemokine; thymus-expressed chemokine
• Gene Symbols: CCL25
• Host Species: Rabbit
• Immunogen: E.coli-derived Recombinant Human TECK (CCL25).
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 6370
• Target Species: Human
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Lyophilized