TEF1 Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific PA5120213

Catalog No. PIPA5120213

Description

Positive test controls include: 293T, HeLa, Mouse skeletal muscle, Mouse heart, Rat heart. The target is usually found in the following locations: Nucleus. Immunogen sequence: APSVPAWQGR SIGTTKLRLV EFSAFLEQQR DPDSYNKHLF VHIGHANHSY SDPLLESVDI RQIYDKFPEK KGGLKELFGK GPQNAFFLVK FWADLNCNIQ D

TEF1 (Transcriptional enhancer factor 1), a member of the TEA/ATTS domain family, is a nuclear protein that is expressed in numerous cell types and plays a role in controlling the expression of numerous genes. TEF family members have a highly conserved DNA-binding domain TEF-1 binds to GT-IIC, SphI/II and M-CAT. TEF-1 also binds to the proximal regulatory element (PRE) of transforming growth factor-alpha, a member of the EGF family that is overexpressed in many types of cancer. Furthermore, TEF-1 represses transcription in placental cells. In vitro, TEF-1 is phosphorylated by several PKC isozymes. TEF-1 is phosphorylated in vivo at serine and threonine residues. Phosphorylation of TEF-1, both in vivo and in vitro, results in a reduction in its DNA-binding capability, which suggests a potential role for TEF-1 in PKC inhibition. TEF-1 also complexes with larger tumor antigen (TAg), and may thus have a role in tumorigenesis. Dimerization of TEF-1 may be important for TEF-1 to function as a regulator of gene transcription.

Specifications

• Antigen: TEF1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Tead1
• Gene Alias: 2610024B07Rik; AA; B230114H05Rik; Gtrgeo5; mTEF-1; N TEAD1; NTEF1; NTEF-1; protein GT-IIC; REF1; Tcf13; TCF-13; TEA domain family member 1; TEA domain family member 1 (SV40 transcriptional enhancer factor); TEA domain transcription factor 1; TEAD1; TEAD-1; TEF1; TEF-1; Transcription factor 13; transcriptional enhancer factor 1; transcriptional enhancer factor TEF-1
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 21676, 361630, 7003
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ChIP Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
• Concentration: 2.16 mg/mL
• Formulation: PBS with 50% glycerol and 0.01% thimerosal; pH 7.3
• Gene Accession No.: P28347, P30051
• Gene Symbols: Tead1
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 200-300 of human TEAD1 (NP_068780.2).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov