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  5. Cytiva TempliPhi™ Sequence Resolver Kit

Cytiva TempliPhi™ Sequence Resolver Kit

Catalog No. 45002266 Shop All Cytiva Products
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No. of Reactions:
20 reactions
200 reactions
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Catalog No. No. of Reactions
45-002-266 200 reactions
45-002-402 20 reactions
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Catalog No. 45-002-266 Supplier Cytiva Supplier No. 28903531
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Description

Includes

Sample buffer, Reaction buffer and Enzyme mix

Uses modified nucleotides in isothermal rolling circle amplification (RCA) to improve results with difficult to sequence templates

  • Cultureless preparation from bacterial colonies, glycerol stock or purified DNA, saves time and reagents
  • Requires 20 minutes hands-on time
  • Excellent sequence finishing performance resolving sequencing stop and compression issues associated with difficult templates
  • Improve difficult template sequencing success with up to 820 bp Phred 20 read length
  • For use directly in cycle sequencing reactions without purification
  • Available in sizes of 20, 50 and 500 reactions

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Store the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°CThaw components on ice and maintain at 0°C to 4°C during handling
Description illustra TempliPhi Sequence Resolver Kit uses the highly processive, strand-displacing Phi29 DNA polymerase and modified nucleotides to amplify circular templates for subsequent sequencing.
For Use With (Application) Amplification of difficult templates for successful DNA sequencing
Includes 1 x 900μL Sample Buffer, 1 x 900μL Reaction Buffer, 1 x 100μL Enzyme mix
No. of Reactions 200 reactions
Product Type illustra TempliPhi Sequence Resolver Kit
Test Time 18 h
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