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  6. Invitrogen™ TER-119 Monoclonal Antibody (TER-119), Super Bright™ 600, eBioscience™, Invitrogen™ 

Invitrogen™ TER-119 Monoclonal Antibody (TER-119), Super Bright™ 600, eBioscience™, Invitrogen™
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Catalog No. p-7114552
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100 μg
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Catalog No. Quantity
63-592-182 100 μg
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Catalog No. 63-592-182 Supplier Invitrogen™ Supplier No. 63592182
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Description

Rat Monoclonal Antibody

Description: The TER-119 monoclonal antibody reacts with mouse erythroid cells from early proerythroblast to mature erythrocyte stages. The TER-119 antigen is present in yolk sac, fetal and newborn liver, but is not expressed by cells carrying BFU-E and CFU-E activities. Several erythroleukemia cell lines tested so far are negative for expression of TER-119 antigen even after dimethylsulfoxide stimulation. Biochemical and molecular analysis of the TER-119 antigen indicate that this molecule is associated with the surface glycophorin A, but is not a typical glycophorin. Applications Reported: This TER-119 antibody has been reported for use in flow cytometric analysis. Applications Tested: This TER-119 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This may be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

TER-119 is a lineage marker for erythroid cells from early proerythroblast to mature erythrocyte stages in adult blood, spleen, and bone marrow. It is also present in yolk sac, and fetal and newborn liver. The TER-119 antigen is not expressed by cells of earlier erythroid development at BFU-e (blast-forming unit erythroid) stage or CFU-e (colony-forming unit erythroid) stage.
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Specifications

Antigen TER-119
Applications Flow Cytometry
Classification Monoclonal
Clone TER-119
Concentration 0.2 mg/mL
Conjugate Super Bright 600
Formulation PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Ly76
Gene Accession No. 0
Gene Alias Ly76; Ly-76; TER119
Gene Symbols Ly76
Host Species Rat
Purification Method Affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 104231
Target Species Mouse
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Liquid
Isotype IgG2b κ
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