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PeproTech TGF alpha Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Catalog No. 500P16BT25
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25 μg
50 μg
500 μg
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Catalog No. Quantity
500P16BT25 25 μg
500P16BT50 50 μg
50-271-8644 500 μg
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Catalog No. 500P16BT25 Supplier PeproTech Supplier No. 500P16BT25UG
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Description

Rabbit Polyclonal Antibody

AA Sequence of recombinant protein: VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLA. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human TGF-alpha. Anti-Human TGF-alpha-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human TGF-alpha by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human TGF-alpha (500-P16) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human TGF-alpha. Western Blot: To detect Human TGF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human TGF-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P16BT-1MG will be provided as 2 x 500 μg

TGF-alpha is an EGF-related polypeptide growth factor that signals through the EGF receptor, and stimulates the proliferation of a wide range of epidermal and epithelial cells. It is produced by monocytes, keratinocytes, and various tumor cells. TGF-alpha induces anchorage-independence transformation in cultured cells. Human, murine and rat TGF-alpha are cross-species reactive. TGFalpha (aa 50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
TRUSTED_SUSTAINABILITY

Specifications

Antigen TGF alpha
Applications ELISA, Western Blot
Classification Polyclonal
Concentration 0.1-1.0 mg/mL
Conjugate Biotin
Formulation PBS with no preservative
Gene TGFA
Gene Accession No. P01135
Gene Alias EGF-like TGF; ETGF; I79_019067; LOC100009150; Protransforming growth factor alpha; RATTGFAA; TFGA; TGF a; TGF type 1; TGF α; TGFA; TGFAA; TGFalpha; TGF-alpha; TGFα; Transforming growth factor; Transforming growth factor alpha; transforming growth factor, alpha; transforming growth factor-alpha; wa1; wa-1; waved 1
Gene Symbols TGFA
Host Species Rabbit
Immunogen E.coli-derived, 5.5 kDa Recombinant Human TGF-alpha.
Purification Method Antigen affinity chromatography
Quantity 25 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 7039
Target Species Human
Content And Storage -20°C
Product Type Antibody
Form Lyophilized
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