Invitrogen™ Themis Monoclonal Antibody (719945)

Rat Monoclonal Antibody

Supplier:  Invitrogen™ MA524337

Catalog No. PIMA524337

Description

In Western blots, 100% cross-reactivity with recombinant human (rh) Themis (aa 2-282) is observed under non-reducing conditions, and approximately 10% cross-reactivity with rhThemis (aa 2-282) is observed under reducing conditions. Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.

Themis is a cytoplasmic protein that is essential for positive selection and the development of single-positive thymocytes. Highest levels of expression are found in CD4+CD8+ double-positive thymocytes, with lower expression found in mature T cells. In addition to Grb1, Themis has been found to associate with PLC gamma 1 and LAT and is quickly phosphorylated by SLP-76 following TCR signaling. There are 5 isoforms of mouse Themis that are produced as a result of alternative splicing.

Specifications

• Antigen: Themis
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 5% trehalose and No Preservative
• Gene Accession No.: Q8BGW0
• Gene Symbols: Themis
• Immunogen: E. coli-derived recombinant mouse Themis Lys122-Lys237.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2b

• Applications: Flow Cytometry, Western Blot
• Clone: 719945
• Conjugate: Unconjugated
• Gene: Themis
• Gene Alias: bA325O24.3; bA325O24.4; C6orf190; C6orf207; E430004N04Rik; FLJ40584; Gasp; GRB2-associated protein; Grb2-associating protein; MGC163388; Protein THEMIS; Protein thylex; RGD1560849; signaling phosphoprotein specific for T cells; SPOT; THEMIS; thylex; thymocyte expressed molecule involved in selection; thymocyte selection associated; thymocyte selection pathway associated; thymocyte-expressed molecule involved in selection; Tsepa
• Host Species: Rat
• Purification Method: Protein A/G
• Regulatory Status: RUO
• Gene ID (Entrez): 210757
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Lyophilized