Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Custom Products and Services
  4. cDNA and ORF Libraries
  5. Thermo Scientific™ ClaSeek™ Library Preparation Kit, Ion Torrent™ Compatible

Thermo Scientific™ ClaSeek™ Library Preparation Kit, Ion Torrent™ Compatible

Catalog No. FERK1351
Change view
Click to view available options
Quantity:
10 Reactions
50 Reactions
2 product options available for selection
Product selection table with 2 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
FERK1351 10 Reactions
FERK1352 50 Reactions
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 2 options available.
2 options
Catalog No. FERK1351 Supplier Thermo Scientific™ Supplier No. K1351

Description

Generate Illumina™-compatible next-gen sequencing libraries from low sample input using a one-tube PCR-free method with optimized master mixes

Thermo Scientific ClaSeek Library Preparation Kit, Illumina™ compatible, is designed for fast and convenient construction of PCR-free NGS fragment library from DNA sample input as low as 500 ng. In combination with the amplification protocol the kit also enables the generation of DNA fragment libraries from ultra-low 5 ng sample input.
The kit utilizes a highly efficient NGS-optimized library construction protocol combining end-conversion and adapter addition steps into a simple and convenient one-tube procedure. This minimizes unnecessary pipetting steps and reduces hands-on time, allowing for PCR-free library construction in less than 70 minutes. The kit is suitable for construction of 50, 300, 500 bp read-length Illumina™-compatible libraries and is validated for use on Illumina™ HiSeq™ and MiSeq™ sequencing platforms.
Highlights
•PCR-free - significantly minimized bias and improved coverage with amplification-free protocol
•Flexible - universal protocol for variable sample input (5 ng - 1 μg) and DNA fragment lengths
•Fast and Convenient - fewer cleanup steps, less hands-on time, optimized master mixes, one-tube end conversion procedure.
Applications
DNA fragment library preparation for downstream use in next generation sequencing workflow.
Includes
• ClaSeek End Conversion Master Mix, IL
• ClaSeek Ligation Mix, IL
• Water, nuclease-free

TRUSTED_SUSTAINABILITY

Specifications

Includes ClaSeek End Conversion Master Mix IT, ClaSeek Ligation Mix IT, Adapters IT, Library Amplification Primer Mix, nuclease-free water, Cleanup Columns (pre-assembled with collection tubes)
Content And Storage Store at -20°C. Cleanup Columns must be stored at room temperature.
For Use With (Application) DNA fragment library preparation for downstream use in next generation sequencing workflow.
Quantity 10 Reactions
Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.