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Thermo Scientific™ Phire Plant Direct PCR Kit (without sampling tools)
Description
Thermo Scientific Phire Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR. The kit is based on Phire Hot Start II DNA Polymerase, a specially engineered enzyme with a DNA binding domain. The unique features of this DNA polymerase make it extremely robust and tolerant of many PCR inhibitors present in plant, fungi, and bacteria.
The Phire Plant Direct PCR Kit includes a complete set of optimized PCR reagents as well as detailed protocols for different sample types, making it an ideal choice for amplification of plant DNA. In addition, the kit includes control primers for positive control reactions. The control primers amplify a highly conserved region of chloroplast DNA and are universal for many plant species.
Features
- No need for time-consuming and expensive DNA purification steps
- Very little sample material required
- Two simple protocols for various applications
- Phire Hot Start II DNA Polymerase provides high yields of specific product with short extension time (20 s/kb)
Applications
- Genotyping
- Transgene detection
Notes
- The optimal annealing temperature for Phire DNA Polymerase may differ significantly from that of Taq-based polymerases.
Specifications
Specifications
| Content And Storage | • Phire Hot Start II DNA Polymerase, 200 μL • 2X Phire Plant Tissue PCR Buffer, 5 x 1.25 mL • Universal Control Primer Mix (25 μM each), 40 μL • Dilution Buffer, 2 x 5 mL Store at -20°C. |
| GC-Rich PCR Performance | Medium |
| PCR Method | Direct PCR |
| Polymerase | Phire Hot Start II DNA Polymerase |
| Reaction Speed | Fast |
| Product Type | Direct PCR Kit |
| Quantity | 500 reactions |
| Shipping Condition | Wet or Dry Ice |
| Hot Start | Built-In Hot Start |
| No. of Reactions | 500 Reactions |
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Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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