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Thermo Scientific™ Maxima H Minus Reverse Transcriptase (200 U/µL)
GSA_VA

A highly thermostable, high processivity reverse transcriptase lacking RNase H activity.

$94.00 - $984.00

Specifications

Concentration 200 U/μL
Final Product Type First-Strand cDNA
Format Tube
GC-Rich PCR Performance High
Reaction Format Separate components
View More Specs

Products 3
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
FEREP0751
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Thermo Scientific™
EP0751
2,000 Units
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FEREP0752
GSA_VA
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10,000 Units
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FEREP0753
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Description

Description

Thermo Scientific Maxima H Minus Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity but lacks RNase H activity due to mutation in RNase H domain of M-MuLV RT. The engineered enzyme features dramatically improved thermostability, 50X higher processivity, robustness and increased synthesis rate compared to wild type M-MuLV RT.
The eliminated RNase H activity enables the enzyme to produce very long RNA transcripts up to 20 kb. Due to its high thermostability, the enzyme maintains full activity during the entire reverse transcription reaction and generates high yields of cDNA. The reaction temperature can be increased up to 65°C for efficient transcription of RNA regions with a high secondary structure or to improve specificity using gene specific primers. The extremely high processivity of Maxima H Minus enzyme results in increased resistance to common reaction inhibitors, such as guanidine, formamide and ethanol.

Highlights

  • Thermostable - 90% active after incubation at 50°C for 60 minutes in a reaction mixture.
  • Active up to 65°C
  • High yields of full-length cDNA up to 20 kb
  • High sensitivity - reproducible cDNA synthesis from a wide range of starting total RNA amounts (1 pg to 5 μg)
  • Efficient - complete cDNA synthesis in 15 to 30 minutes
  • Increased resistance to common reaction inhibitors
  • Incorporates modified nucleotides
Applications
  • First strand cDNA synthesis for RT-PCR and real-time RT-PCR.
  • Reverse transcription at elevated temperatures to reduce effects of secondary structure.
  • Synthesis of cDNA for cloning and expression.
  • Generation of labeled cDNA probes for microarrays.
  • Analysis of RNA by primer extension.
Includes
Maxima H Minus Reverse Transcriptase is supplied with 5X RT Buffer (250 mM Tris-HCl (pH 8.3 at 25°C), 375 mM KCl, 15 mM MgCl2, 50 mM DTT).

Note
Also available: Maxima H Minus First Strand cDNA Synthesis Kit.

Order Info

Orders placed Monday through Wednesday ship next day; Thursday and Friday orders ship the following Monday.

Specifications

Specifications

200 U/μL
Tube
Separate components
Reverse Transcription
RNA
Maxima H Minus
First-Strand cDNA
High
15 to 30 min.
Up to 20 kb
50°C to 55°C
Reduced
SDS
Product Certifications
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