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  5. Thermo Scientific™ Pierce™ ECL 2 Western Blotting Substrate 

Thermo Scientific™ Pierce™ ECL 2 Western Blotting Substrate
GSA_VA

Catalog No. PI80196
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Quantity:
25 mL
100 mL
3 x 100 mL
Sufficient For:
10 Mini-blots/1000 cm^2 of membrane
250 cm^2 of membrane
30 Mini-blots/3000 cm^2 of membrane
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Catalog No. Quantity Sufficient For
PI80196 100 mL 10 Mini-blots/1000 cm^2 of membrane
PI80196X3 3 x 100 mL 30 Mini-blots/3000 cm^2 of membrane
PI80197 25 mL 250 cm^2 of membrane
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Catalog No. PI80196 Supplier Thermo Scientific™ Supplier No. 80196
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Description

Includes

Detection Reagent 1 and 2

Recommended HRP substrate for fluorescence imagers

Pierce ECL 2 Substrate is a chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated antibodies and probes by Western blotting using X-ray film, CCD camera documentation systems or laser-based imagers. Pierce ECL 2 Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers.

Pierce ECL 2 substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. A broad dynamic range and excellent sensitivity combined with a long-lasting signal make Pierce ECL 2 Substrate a desirable Western blotting substrate.

Highlights:

  • High sensitivity — detect targets down to the low-picogram level
  • Long signal duration — sustained light output for as long as five hours
  • More imaging options — X-ray, CCD or laser-based imagers
  • More affordable — high quality and performance at a lower price than other suppliers' similar substrates

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TRUSTED_SUSTAINABILITY

Specifications

Product Type ECL Western Blot Substrate
Quantity 100 mL
Sufficient For 10 Mini-blots/1000 cm^2 of membrane
Kit Contents Detection reagent 1 and 2
Content And Storage 2°C to 8°C

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov
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