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Thermo Scientific™ Pierce™ ECL 2 Western Blotting Substrate
Description
Includes
Detection Reagent 1 and 2
Pierce ECL 2 Substrate is a chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated antibodies and probes by Western blotting using X-ray film, CCD camera documentation systems or laser-based imagers. Pierce ECL 2 Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers.
Pierce ECL 2 substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. A broad dynamic range and excellent sensitivity combined with a long-lasting signal make Pierce ECL 2 Substrate a desirable Western blotting substrate.
Highlights:
- High sensitivity — detect targets down to the low-picogram level
- Long signal duration — sustained light output for as long as five hours
- More imaging options — X-ray, CCD or laser-based imagers
- More affordable — high quality and performance at a lower price than other suppliers' similar substrates
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Specifications
Specifications
| Product Type | ECL Western Blot Substrate |
| Quantity | 100 mL |
| Sufficient For | 10 Mini-blots/1000 cm^2 of membrane |
| Kit Contents | Detection reagent 1 and 2 |
| Content And Storage | 2°C to 8°C |
Safety and Handling
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