Thermo Scientific™ Pierce™ ECL 2 Western Blotting Substrate

Recommended HRP substrate for fluorescence imagers

$90.78 - $644.28

Specifications

Kit Contents Detection reagent 1 and 2
Storage Requirements 2° to 8°C
Product Type Pierce ECL 2 western blotting Substrate
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Includes : Detection Reagent 1 and 2

    Specifications

    Kit Contents Detection reagent 1 and 2
    Storage Requirements 2° to 8°C
    Product Type Pierce ECL 2 western blotting Substrate

    Pierce ECL 2 Substrate is a chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated antibodies and probes by Western blotting using X-ray film, CCD camera documentation systems or laser-based imagers. Pierce ECL 2 Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers.

    Pierce ECL 2 substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. A broad dynamic range and excellent sensitivity combined with a long-lasting signal make Pierce ECL 2 Substrate a desirable Western blotting substrate.

    Highlights:

    • High sensitivity — detect targets down to the low-picogram level
    • Long signal duration — sustained light output for as long as five hours
    • More imaging options — X-ray, CCD or laser-based imagers
    • More affordable — high quality and performance at a lower price than other suppliers' similar substrates

  • Products 3
  • Description & Specifications
Products
Catalog Number Mfr. No. Sufficient For Quantity Price Quantity & Availability    
PI80196 THERMO SCI PIERCE BIOTECH
80196
10 Mini-gel blots, 1000cm2 of membrane 100mL Each for $316.68
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PI80196X3 THERMO SCI PIERCE BIOTECH
80196X3
30 Mini-gel blots, 3000cm2 of membrane 3 x 100mL Each for $644.28
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PI80197 THERMO SCI PIERCE BIOTECH
80197
250cm2 of membrane 25mL Each for $90.78
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Descriptions & Specifications

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