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Thermo Scientific™ Pierce™ IQ Assay Kits
Description
Includes
Includes:
- Dye-labeled substrate
- IQ Reagent A
- IQ Reagent B
Quantify kinase or phosphatase activity by direct measurement of the phosphorylation state of a fluorophore-labeled peptide substrate. Phosphorylation is measured by the change in fluorescence intensity that occurs when a proprietary iron-containing compound binds specifically to phosphoryl groups on the peptides. The change in fluorescence is proportional to the extent of phosphorylation of the fluorophore-labeled peptide, because the iron-containing compound quenches the fluorescent emission exclusively on the phosphorylated form of the fluorophore-labeled peptide.
- Homogeneous, in-solution assay format does not require wash steps or beads
- Universal system for any kinase or phosphatase—no antibody required
- Assay is directly scalable from a 96-well format to 384- and 1536-well formats
- Robust results—Z« of 0.6 for 10% conversion of substrate in a kinase reaction and Z« of 0.7 for 10% conversion of substrate in a phosphatase reaction
- Addition of IQ reagents stops the enzyme reaction, so plates may be read immediately or up to two days later
- Results can be read on any fluorometer
†Indicates number of assays performed in a 96-well format.
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