Thermo Scientific™ RapidOut DNA Removal Kit

Description

Includes

• DNase I, RNase-free (1U/μL) - 250Lμ
• 10X Reaction Buffer with MgCl₂ - 1mL
• DNase Removal Reagent (DRR) - 500μL
• Water, nuclease-free - 2 x 1.25mL

Thermo Scientific RapidOut DNA Removal Kit rapidly and safely removes genomic DNA from total RNA and mRNA preparations.

Thermo Scientific RapidOut DNA Removal Kit rapidly and safely removes genomic DNA from total RNA and mRNA preparations. Complete digestion of DNA and safe removal of DNase I from the digestion reaction is ensured without RNA damaging steps, such as heating or organic extraction. First, the RNA sample is treated with recombinant RNase-free DNase I to levels below the limit of detection by routine PCR. DNase I is safely removed subsequently using proprietary DNase Removal Reagent (DRR).

DRR efficiently binds DNase I and the complex is collected at the bottom of the tube by centrifugation. The purified RNA is collected as a supernatant. The RNA after RapidOut procedure is free from DNA contamination and free of DNase I. It is ready to use in different applications including end-point or real-time RT-PCR, cloning, microarrays, and Northern blotting.

Highlights
• Efficient—complete ds and ssDNA digestion and proprietary technology for DNase I removal
• Rapid—single step sufficient for complete DNase I removal
• Safe—no need for toxic organic extractions or RNA-damaging heating steps

Main Applications
• RNA isolation and RNA analysis, particularly RT-qPCR and RT-PCR (customers performing expression analysis of low transcription level genes.
• Customers performing ds-cDNA synthesis from total RNA preps.

Other applications
• Elimination of DNA from RNA for microinjections and transfection.
• Elimination of DNA from RNA prior microarray analysis.
• Elimination of DNA from RNA prior Northern blot analysis.

Specifications

• Enzyme: DNase
• Quantity: 50 preps
• Product Type: Removal Kit

For Research Use Only. Not for use in diagnostic procedures.