Thermo Scientific™ RapidOut DNA Removal Kit

Catalog No.	Quantity
FERK2981	50 preps

Catalog No. FERK2981 Supplier Thermo Scientific™ Supplier No. K2981

Description

Includes

• DNase I, RNase-free (1U/μL) - 250Lμ
• 10X Reaction Buffer with MgCl₂ - 1mL
• DNase Removal Reagent (DRR) - 500μL
• Water, nuclease-free - 2 x 1.25mL

Thermo Scientific RapidOut DNA Removal Kit rapidly and safely removes genomic DNA from total RNA and mRNA preparations.

Thermo Scientific RapidOut DNA Removal Kit rapidly and safely removes genomic DNA from total RNA and mRNA preparations. Complete digestion of DNA and safe removal of DNase I from the digestion reaction is ensured without RNA damaging steps, such as heating or organic extraction. First, the RNA sample is treated with recombinant RNase-free DNase I to levels below the limit of detection by routine PCR. DNase I is safely removed subsequently using proprietary DNase Removal Reagent (DRR).

DRR efficiently binds DNase I and the complex is collected at the bottom of the tube by centrifugation. The purified RNA is collected as a supernatant. The RNA after RapidOut procedure is free from DNA contamination and free of DNase I. It is ready to use in different applications including end-point or real-time RT-PCR, cloning, microarrays, and Northern blotting.

Highlights
• Efficient—complete ds and ssDNA digestion and proprietary technology for DNase I removal
• Rapid—single step sufficient for complete DNase I removal
• Safe—no need for toxic organic extractions or RNA-damaging heating steps

Main Applications
• RNA isolation and RNA analysis, particularly RT-qPCR and RT-PCR (customers performing expression analysis of low transcription level genes.
• Customers performing ds-cDNA synthesis from total RNA preps.

Other applications
• Elimination of DNA from RNA for microinjections and transfection.
• Elimination of DNA from RNA prior microarray analysis.
• Elimination of DNA from RNA prior Northern blot analysis.

Specifications

• Enzyme: DNase
• Quantity: 50 preps
• Product Type: Removal Kit

Frequently Asked Questions (FAQs)

Does DRR (DNase Binding Reagent) from RapidOut DNA Removal Kit bind to RNA present in enzymatic reaction mixtures? What is the recovery of RNA after the procedure?

RNA recovery after the RapidOut DNA removal procedure is high. Typically it is higher than conventional DNAse I inactivation methods such as heat, EDTA or chloroform/phenol extraction.

Does the DRR (DNase Binding Reagent) from RapidOut DNA Removal Kit bind to other proteins? Is the kit suitable for removal of other proteins from the reaction mixture?

We have tested the kit for DNase I removal only. Although DRR can bind to other proteins, we cannot provide any recommendations for removal of protein contaminants from the enzymatic mixtures. If the RNA sample contains a considerable amount of protein contaminants, modify the RNA purification protocol to reduce protein contamination.

For Research Use Only. Not for use in diagnostic procedures.