Thermo Scientific™ RevertAid RT Reverse Transcription Kit

Description

The Thermo Scientific RevertAid RT Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates.

The Thermo Scientific RevertAid RT Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates. The kit uses RevertAid Reverse Transcriptase (RT), which has lower RNase H activity compared to AMV reverse transcriptase. The enzyme maintains activity at 42–50°C and is suitable for synthesis of cDNA up to 13 kb. The recombinant RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA from degradation at temperatures up to 55°C.

First strand cDNA synthesized with this system can be directly used as a template in PCR or real-time PCR. It is also ideal as a first step for second strand cDNA synthesis or linear RNA amplification. Radioactively and non-radioactively labeled nucleotides can be incorporated into first strand cDNA for use as a probe in hybridization experiments, including microarrays.

Features of the RevertAid RT Kit include:
• Full-length first strand cDNA up to 13 kb
• Optimum reaction temperature 42°C
• Complete kit includes all the components for RT reactions

Applications
• First strand cDNA synthesis for RT-PCR and RT-qPCR
• Construction of full length cDNA libraries
• Antisense RNA synthesis

Order Info

Orders placed Monday through Wednesday ship next day; Thursday and Friday orders ship the following Monday.

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Specifications

• Content And Storage:

  • RevertAid Reverse Transcriptase
  • Ribolock RNase Inhibitor
  • 5X Reaction Buffer
  • dNTP Mix
  • Random Hexamer Primer
  • Nuclease-free water
  
  Store at –20°C.
  

  
  
• Format: Kit
• Reaction Speed: 60 min.
• Technique: Reverse Transcription
• Optimal Reaction Temperature: 42°C
• Quantity: 500 reactions
• Reverse Transcriptase: RevertAid
• Ribonuclease H Activity: Yes
• Shipping Condition: Dry Ice
• For Use With (Application): Real Time PCR (qPCR)
• Final Product Type: First-Strand cDNA
• No. of Reactions: 500 Reactions
• Reaction Format: Separate components
• Reagent Type: Reverse Transcription
• Size (Final Product): Up to 13 kb
• Starting Material: RNA

For Research Use Only. Not for use in diagnostic procedures.