Thermo Scientific RevertAid RT Reverse Transcription Kit
A complete system for efficient synthesis of first strand cDNA from RNA templates.
Manufacturer: Thermo Scientific K1691
The kit uses RevertAid Reverse Transcriptase which has lower RNase H activity, compared to AMV reverse transcriptase. The recombinant RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA template from degradation. It is fully compatible with reverse transcription reaction, as it maintains activity at temperatures up to 55°C. The oligo(dT)18 anneals selectively on the poly(A) tail of mRNA. Random hexamer primers do not require the presence of poly(A). Therefore, they can be used for transcription of the 5'-end regions of mRNA or cDNA synthesis using RNA without poly(A) tail, e.g., micro RNAs. Gene-specific primers may also be used with the kits. The first strand of cDNA can be directly used as a template in PCR (see RT-PCR protocol), real-time PCR or in second strand cDNA synthesis (see protocol).
Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit is suitable for synthesis of cDNA up to 13 kb. RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA templates from degradation.
The RevertAid First Strand cDNA Synthesis Kit is supplied with both oligo(dT)18 and random hexamer primers. The oligo(dT)18 primer anneals selectively to the poly(A) tail of mRNA. Random hexamer primers do not require the presence of the poly(A) tail, therefore, they can be used for transcription of the 5'-end regions of mRNA or cDNA synthesis of RNA species lacking a poly(A) tail (e.g., microRNAs). Gene-specific primers may also be used with the kit.
· Full-length first strand cDNA up to 13 kb
· Optimum reaction temperature 42°C
· Complete kit - all the components for the RT reaction are included
· First strand cDNA synthesis for RT-PCR and RT-qPCR
· Construction of full length cDNA libraries
· Antisense RNA synthesis
The RevertAid First Strand cDNA Synthesis Kit contains RevertAid Reverse Transcriptase, RiboLock RNase Inhibitor, 5X Reaction Buffer, dNTP Mix, Random Hexamer Primer, Control GAPDH RNA, 10 µM Forward GAPDH Primer, 10 µM Reverse GAPDH Primer, and nuclease-free water.
The RevertAid RT Kit contains RevertAid Reverse Transcriptase, RiboLock Rnase Inhibitor, 5X Reaction Buffer, dNTP Mix, Random Hexamer Primer, and nuclease-free water.
>Quality Control:The kit is functionally tested in RT-PCR using 100 fg control GAPDH RNA and GAPDH control primers generated a 496 bp product visible on agarose gel after ethidium bromide staining.
> Storage Condition:-20°C
First strand cDNA synthesis for RT-PCR and real-time RT-PCR (1, 2); Construction of full length cDNA libraries; Generation of probes for hybridization; aRNA synthesis.
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|The RevertAid RT Kit contains:
• RevertAid Reverse Transcriptase
• RiboLock RNase Inhibitor
• 5X Reaction Buffer
• dNTP Mix
• Random Hexamer Primer
• Nuclease-free water
Store at -20°C.
|Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR)|
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