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Thermo Scientific™ RevertAid RT Reverse Transcription Kit

The Thermo Scientific RevertAid RT Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates.

Supplier:  Thermo Scientific™ K1691

Catalog No. FERK1691

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The Thermo Scientific RevertAid RT Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates. The kit uses RevertAid Reverse Transcriptase (RT), which has lower RNase H activity compared to AMV reverse transcriptase. The enzyme maintains activity at 42–50°C and is suitable for synthesis of cDNA up to 13 kb. The recombinant RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA from degradation at temperatures up to 55°C.

First strand cDNA synthesized with this system can be directly used as a template in PCR or real-time PCR. It is also ideal as a first step for second strand cDNA synthesis or linear RNA amplification. Radioactively and non-radioactively labeled nucleotides can be incorporated into first strand cDNA for use as a probe in hybridization experiments, including microarrays.

Features of the RevertAid RT Kit include:
• Full-length first strand cDNA up to 13 kb
• Optimum reaction temperature 42°C
• Complete kit includes all the components for RT reactions

• First strand cDNA synthesis for RT-PCR and RT-qPCR
• Construction of full length cDNA libraries
• Antisense RNA synthesis

Order Info

Orders placed Monday through Wednesday ship next day; Thursday and Friday orders ship the following Monday.



• RevertAid Reverse Transcriptase
• Ribolock RNase Inhibitor
• 5X Reaction Buffer
• dNTP Mix
• Random Hexamer Primer
• Nuclease-free water

Store at –20°C.

60 min.
Real Time PCR (qPCR)
500 Reactions
Reverse Transcription
Reverse Transcription
Dry Ice
First-Strand cDNA
Separate components
Up to 13 kb


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