Thermo Scientific™ RevertAid RT Reverse Transcription Kit
The Thermo Scientific RevertAid RT Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates.
Supplier: Thermo Scientific™ K1691
The Thermo Scientific RevertAid RT Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates. The kit uses RevertAid Reverse Transcriptase (RT), which has lower RNase H activity compared to AMV reverse transcriptase. The enzyme maintains activity at 42–50°C and is suitable for synthesis of cDNA up to 13 kb. The recombinant RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA from degradation at temperatures up to 55°C.
First strand cDNA synthesized with this system can be directly used as a template in PCR or real-time PCR. It is also ideal as a first step for second strand cDNA synthesis or linear RNA amplification. Radioactively and non-radioactively labeled nucleotides can be incorporated into first strand cDNA for use as a probe in hybridization experiments, including microarrays.
Features of the RevertAid RT Kit include:
• Full-length first strand cDNA up to 13 kb
• Optimum reaction temperature 42°C
• Complete kit includes all the components for RT reactions
• First strand cDNA synthesis for RT-PCR and RT-qPCR
• Construction of full length cDNA libraries
• Antisense RNA synthesis
Orders placed Monday through Wednesday ship next day; Thursday and Friday orders ship the following Monday.
• RevertAid Reverse Transcriptase
|Real Time PCR (qPCR)|
|Up to 13 kb|
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For Research Use Only. Not for use in diagnostic procedures.