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Thermo Scientific™ T4 beta-glucosyltransferase

Catalog No. FEREO0831
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500 units
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FEREO0831 500 units
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Catalog No. FEREO0831 Supplier Thermo Scientific™ Supplier No. EO0831
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Description

Highly specific and fast T4 β-glucosyltransferase for 5-hmC modification analysis

T4 β-glucosyltransferase (T4 BGT) transfers the glucose moiety of uridine diphosphoglucose (UDP-glucose) to the 5-hydroxymethylcytosine (5-hmC) residues in double-stranded DNA generating β-glucosyl-5 hydroxymethylcytosine.

Thermo Scientific T4 BGT is specifically formulated for fast reaction times without compromising the reaction efficiency. The enzyme completes 5-hmC glucosylation of 1ωg DNA at 37°C in 15 minutes.

Highlights

  • Specific – selectively transfers glucose to the hydroxymethyl moiety of 5-hmC
  • Fast – complete glucosylation of 1μg DNA in just 15 min
  • Convenient – supplied with optimized buffer and UDP-glucose
Applications
  • Locus specific detection of 5-hmC
  • Enrichment of 5-hmC containing DNA
  • Labeling of 5-hmC residues using a radioactive UDP-glucose donor
Includes
  • T4 β-glucosyltransferase, 5 U/μL
  • 10X Epi buffer
  • 10X UDP-glucose

TRUSTED_SUSTAINABILITY

Specifications

Enzyme T4 β-glucosyltransferase
Compatible Buffer 10X Epi Buffer
Quantity 500 units
Product Type T4 Beta Glucosyltransferase

For Research Use Only. Not for use in diagnostic procedures.

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