Invitrogen™ ThPOK Monoclonal Antibody (2POK), PE, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Supplier:  Invitrogen™ 12592882

Catalog No. 50-112-2355

Description

Description: This 2POK monoclonal antibody reacts with mouse ThPOK, which is also known as c-Krox and Zbtb7b. In hematopoietic cells, this 78 kDa transcription factor is expressed in thymocytes and T lymphocytes in the spleen and lymph nodes. In non-hematopoietic cells, ThPOK can be detected in the skin, heart, smooth muscle, and liver. Studies demonstrate that ThPOK promotes CD4+ lineage differentiation by inhibiting the expression of CD8 lineage-specific genes such as Runx3. ThPOK has also been shown to inhibit Cd4 silencing, another key facet required for differentiation to the CD4+ lineage. In addition to its role in thymocyte development, ThPOK is involved in the effector functions of invariant natural killer T (iNKT) cells and gamma delta T cells. Applications Reported: This 2POK antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This 2POK antibody has been tested by intracellular staining and flow cytometric analysis of mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

ZFP67 is an early growth response gene that encodes a zinc finger-containing transcription factor that binds to the promoter regions of type I collagen genes (e.g., COL1A1; MIM 120150) and has a role in development.

Specifications

• Antigen: ThPOK
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q64321
• Gene Symbols: Zbtb7b
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 22724
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: 2POK
• Conjugate: PE
• Gene: Zbtb7b
• Gene Alias: CKROX; c-KROX; DKFZp686G01254; hcKROX; Krueppel-related zinc finger protein cKrox; RP11-307C12.9; T helper-inducing POZ/Krueppel factor; T-helper-inducing POZ/Krueppel-like factor; Thpok; Vzinc finger and BTB domain containing 7B; ZBTB15; ZBTB7B; ZFP67; Zfp-67; zinc finger and BTB domain containing 15; zinc finger and BTB domain containing 7B; Zinc finger and BTB domain-containing protein 15; Zinc finger and BTB domain-containing protein 7B; zinc finger protein 67; Zinc finger protein 67 homolog; zinc finger protein 857B; Zinc finger protein Th-POK; ZNF857B
• Host Species: Rat
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a κ

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only.