Novus Biologicals™ Tie-2 Antibody Pair [HRP]

Description

Polyclonal/Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes an antibody specific for Human Tie-2.

Specifications

• Assay Range: 78.125 pg/mL
• Assay Sensitivity: 78.125-5000 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes an antibody specific for Human Tie-2.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: angiopoietin-1 receptor, CD202B, CD202b antigen, EC 2.7.10, EC 2.7.10.1, hTIE2, p140 TEK, soluble TIE2 variant 1, soluble TIE2 variant 2, TEK tyrosine kinase, endothelial, TIE-2, TIE2CD202b, Tunica interna endothelial cell kinase, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, venous malformations, multiple cutaneous and mucosal, VMCM, VMCM1
• Gene ID (Entrez): 7010
• Gene Symbol: TEK
• Host Species: Rabbit
• Kit Contents:
  Standard: Each vial contains 245 ng of recombinant Human Tie-2.
  Capture Antibody: 1.5 mg/mL of rabbit anti-Human Tie-2 monoclonal antibody (in PBS, pH 7.4).
  Detection Antibody: 0.2 mg/mL of rabbit anti-Human Tie-2 polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Angiogenesis, Cancer, Phospho Specific, Protein Kinase
• Storage Requirements: Storage is content dependent.
• Target: Tie-2
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 5000 pg/mL is recommended.