Invitrogen™ TIGIT Monoclonal Antibody (GIGD7), Functional Grade, eBioscience™

Catalog No.	Quantity
16-950-185	500 μg

Catalog No. 16-950-185 Supplier Invitrogen™ Supplier No. 16950185

Description

Rat Monoclonal Antibody

Description: The GIGD7 monoclonal antibody reacts with mouse T cell immunoreceptor with Ig and ITIM domains (TIGIT, VSTM3, WUCAM), a 26 kDa protein that is a member of the poliovirus receptor (PVR) family. In mice, the expression of TIGIT has been reported in follicular T helper cells, while in humans, the expression of TIGIT has been reported on NK cells, regulatory T cells, follicular T helper cells, memory CD4+ T cells, and CD8+ T cells. TIGIT is not expressed on B cells or naive CD4+ T cells. TIGIT is upregulated on CD4+ T cells following activation. TIGIT can interact with certain members of the PVR and PVR-like families, including CD155, and also mediates the interaction of NK cells and T cells with antigen presenting cells, fibroblasts and endothelial cells that express PVR and PVR-like proteins. Applications Reported: This GIGD7 antibody has been reported for use in flow cytometric analysis. (Fluorochrome-conjugated GIGD7 is recommended for use in flow cytometry). Applications Tested: This GIGD7 antibody has been tested by flow cytometric analysis of mouse TIGIT-transfected cell lines. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount ( μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Sto...

TIGIT is a 26 kDa protein that belongs to the poliovirus receptor (PVR) family. It is expressed on NK cells, regulatory T cells, follicular T helper cells, memory CD4+ T cells, and CD8+ T cells, but not on B cells or naive CD4+ T cells. However, TIGIT can be upregulated on naive CD4+ T cells upon activation. TIGIT interacts with members of the PVR and PVR-like families, including CD155. This interaction mediates the engagement of NK and T cells with antigen-presenting cells, fibroblasts, and endothelial cells expressing PVR and PVR-like proteins. Binding of TIGIT to PVR leads to increased secretion of IL-10 and decreased secretion of IL-12B, promoting the generation of mature immunoregulatory dendritic cells and suppressing T cell activation. Through these interactions, TIGIT plays a significant role in modulating immune responses and maintaining immune homeostasis.

Specifications

• Antigen: TIGIT
• Applications: Flow Cytometry, Western Blot, Functional Assay
• Classification: Monoclonal
• Clone: GIGD7
• Concentration: 1 mg/mL
• Conjugate: Functional Grade
• Formulation: PBS with no preservative; pH 7.2
• Gene: TIGIT
• Gene Accession No.: P86176
• Gene Alias: ENSMUSG00000071552; T cell immunoreceptor with Ig and ITIM domains; T-cell immunoreceptor with Ig and ITIM domains; TIGIT; V-set and immunoglobulin domain containing 9; V-set and immunoglobulin domain-containing protein 9; V-set and transmembrane domain containing 3; V-set and transmembrane domain-containing protein 3; VSIG9; VSTM3; Washington University cell adhesion molecule; WUCAM
• Gene Symbols: TIGIT
• Host Species: Rat
• Immunogen: mTIGIT-Fc treated with 4% PFA.
• Purification Method: Affinity chromatography
• Quantity: 500 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 100043314, 363784
• Target Species: Mouse, Rat
• Content And Storage: 4°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2a κ

Safety and Handling

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