TIGIT Monoclonal Antibody (GIGD7), PE, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-112-2442	LIFE TECHNOLOGIES 12950180	25 μg
50-112-9360	LIFE TECHNOLOGIES 12950182	100 μg

Description

Description: The GIGD7 monoclonal antibody reacts with mouse T cell immunoreceptor with Ig and ITIM domains (TIGIT, VSTM3, WUCAM), a 26 kDa protein that is a member of the poliovirus receptor (PVR) family. In mice, the expression of TIGIT has been reported in follicular T helper cells, while in humans, the expression of TIGIT has been reported on NK cells, regulatory T cells, follicular T helper cells, memory CD4+ T cells, and CD8+ T cells. TIGIT is not expressed on B cells or naive CD4+ T cells. TIGIT is upregulated on CD4+ T cells following activation. TIGIT can interact with certain members of the PVR and PVR-like families, including CD155, and also mediates the interaction of NK cells and T cells with antigen presenting cells, fibroblasts and endothelial cells that express PVR and PVR-like proteins. Applications Reported: This GIGD7 antibody has been reported for use in flow cytometric analysis. Applications Tested: This GIGD7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells.

Specifications

• Antigen: TIGIT
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: ENSMUSG00000071552; T cell immunoreceptor with Ig and ITIM domains; T-cell immunoreceptor with Ig and ITIM domains; TIGIT; V-set and immunoglobulin domain containing 9; V-set and immunoglobulin domain-containing protein 9; V-set and transmembrane domain containing 3; V-set and transmembrane domain-containing protein 3; VSIG9; VSTM3; Washington University cell adhesion molecule; WUCAM
• Gene Symbols: TIGIT
• Isotype: IgG2a κ
• Purification Method: Affinity Chromatography
• Product Type: Antibody

• Clone: GIGD7
• Applications: Flow Cytometry
• Conjugate: PE
• Host Species: Rat
• Target Species: Mouse
• Gene Accession No.: P86176
• Gene ID (Entrez): 100043314
• Immunogen: mTIGIT-Fc treated with 4% PFA.
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Gene: TIGIT

For Research Use Only.