Invitrogen™ TLR2 Monoclonal Antibody (mT2.4), FITC

Catalog No.	Quantity
FERMA549110	100 μg

Catalog No. FERMA549110 Supplier Invitrogen™ Supplier No. MA549110

Description

Mouse Monoclonal Antibody

The monoclonal antibody mT2.4 inhibits murine TLR2-mediated cell activation. It masks the lipopeptide binding site in the recombinant extracellular domain of TLR2 (TLR2ECD) and does not hinder the receptor complex formation. The antibody reacts with the native TLR2 protein and not with denatured TLR2 protein . Furthermore the antibody stains overexpressed, as well as endogenous cell surface- and intracellular mouse TLR2.

TLR2 is a member of the Toll-like receptor (TLR) family which play a fundamental role in pathogen recognition and activation of innate immunity. TLR proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) alpha/beta and NF-kappa-B. TLR2 can form heterodimers with either TLR1 or TLR6, and as a heterodimer, can recognize a variety of bacterial and mycoplasma lipoproteins respectively. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. The various TLRs exhibit different patterns of expression. TLR2 is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. TLR2 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. Ten human homologs of TLRs (TLR1-10) have been described. TLR2 is expressed in human cells such as tonsils, microglia, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes express the highest level of TLR2 followed by CD15+ granulocytes, CD19+ B-cells, and CD3+ T-cells. The expression of TLR2 on different cell types are regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes.

Specifications

• Antigen: TLR2
• Applications: Flow Cytometry, Functional Assay, Inhibition Assays, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
• Classification: Monoclonal
• Clone: mT2.4
• Concentration: 100 μg/mL
• Conjugate: FITC
• Formulation: PBS with 1% BSA and 0.02% sodium azide
• Gene: Tlr2
• Gene Accession No.: Q9QUN7
• Gene Alias: CD282; Ly105; TIL4; Tlr2; TLR-2; toll like receptor 2; toll/interleukin-1 receptor-like protein 4; Toll-like receptor; toll-like receptor 2; toll-like receptor 2 variant 1; toll-like receptor 2 variant 2; toll-like-receptor 2, exon 1a_; Toll-like-receptor, exon 1b; Toll-like-receptor, exon 1b_; Toll-like-receptor2, exon 1a
• Gene Symbols: Tlr2
• Host Species: Mouse
• Immunogen: Monoclonal antibody mT2.4 reacts with mouse Toll-like receptor 2 (TLR2, CD282).
• Purification Method: Protein G
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 24088
• Target Species: Mouse
• Content And Storage: 4°C, store in dark
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2b