Invitrogen™ Human TNF alpha Matched Antibody Pair

Catalog No.	Quantity
50-179-27	10 x 96 Tests

Catalog No. 50-179-27 Supplier Invitrogen™ Supplier No. BMS2034MST

Description

ELISA

The Human TNF-alpha Matched Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human TNF-alpha. Buffer reagents and microplates needed to complete the ELISA reaction are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

Specifications

• Antigen: TNF alpha
• Applications: ELISA
• Classification: Monoclonal/Polyclonal
• Conjugate: Biotin
• Gene Accession No.: P01375
• Gene Alias: Cachectin, Tumor necrosis factor alpha, DIF
• Gene Symbols: TNF
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 7124
• Target Species: Human
• Content And Storage: 1 vial (2.2 mL) monoclonal Coating Antibody to human TNF-alpha total (100 ug/mL)
  1 vial (55 uL) Biotin-Conjugate anti-human TNF-alpha total polyclonal antibody
  1 vial (22 uL) Streptavidin-HRP
  1 vial human TNF-alpha total Standard protein lyophilized, 30 ng/mL upon reconstitution dilution
  2 vials (50 mL) Sample Diluent
• Product Type: ELISA Kit

Safety and Handling

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