Novus Biologicals™ TNF-alpha Antibody Pair [HRP]

Description

Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Ferret TNF-alpha.

Specifications

• Assay Range: 156.25 pg/mL
• Assay Sensitivity: 156.25-5000 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Ferret TNF-alpha.
• Target Species: Ferret
• For Use With (Application): Sandwich ELISA
• Gene Alias: APC1 protein, Cachectin, DIF, TNF, monocyte-derived, TNF-a, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
• Gene ID (Entrez): 7124
• Gene Symbol: TNF
• Host Species: Mouse/Rabbit
• Kit Contents:
  Standard: Each vial contains 70 ng of recombinant TNF-alpha. Reconstitute with 1 mL detection antibody dilution buffer.
  Capture Antibody: 0.5 mg/mL of rabbit anti-TNF-alpha monoclonal antibody (in PBS, pH 7.4).
  Detection Antibody: 0.2 mg/mL mouse anti-TNF-alpha monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Adaptive Immunity, Asthma, Autophagy, Biologically Active Proteins, Cancer, Cytokine Research, Diabetes Research, Immune System Diseases, Immunology, Innate Immunity
• Storage Requirements: Storage is content dependent.
• Target: TNF-alpha
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: After reconstitution, store at -20 to -80°C in a manual defrost freezer. A six-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 5 ng/mL is recommended.