Novus Biologicals™ TNF-alpha Antibody Pair [HRP]

Description

Mouse Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human TNF-alpha.

Specifications

• Assay Range: 23.44 pg/mL
• Assay Sensitivity: 12.5-800 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human TNF-alpha.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: APC1 protein, Cachectin, DIF, TNF, monocyte-derived, TNF-a, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
• Gene ID (Entrez): 7124
• Gene Symbol: TNF
• Host Species: Mouse
• Kit Contents:
  Detection Antibody: 0.2 mg/mL of mouse anti-Human TNF-alpha monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
  Capture Antibody: 0.5 mg/mL of mouse anti-Human TNF-alpha monoclonal antibody (in PBS, pH 7.4).
  Standard: Each vial contains 38 ng of recombinant Human TNF-alpha.
• Quantity: 5 Plates
• Regulatory Status: RUO
• Research Discipline: Adaptive Immunity, Asthma, Autophagy, Biologically Active Proteins, Cancer, Cytokine Research, Diabetes Research, Immune System Diseases, Immunology, Innate Immunity
• Storage Requirements: Storage is content dependent.
• Target: TNF-alpha
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 1.2 μg/mL in Dilution Buffer before use. 3. Standard: Reconstitute with 1 ml Dilution Buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in Dilution Buffer, and a high standard of 800 pg/mL is recommended.