Invitrogen™ TNF alpha Monoclonal Antibody (2TNF-H33), Biotin

Catalog No.	Quantity
ENM302B	250 μg

Catalog No. ENM302B Supplier Invitrogen™ Supplier No. M302B

Description

Mouse Monoclonal Antibody

M302B targets TNF Alpha in ELISA, and WB applications and shows reactivity with Human samples. The M303 antibody (clone 2TNF-H34A) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M302B (biotinylated conjugate of clone 2TNG-H33). The M302B immunogen is recombinant human TNF alpha. M302B detects TNF Alpha which has a predicted molecular weight of approximately 26 kDa. The M302B TNFa antibody (biotinylated conjugate of clone 2TNF-H33) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody M303 (clone 2TNF-H34A) Typical dilutions for sandwich ELISA include 1 μg/mL for coating and 0.125 - 0.25 μg/mL for detection. Biotinylated antibody M302B (clone 2TNF-H33) and antibody M303 (clone 2TNF-H34A) have successfully been used in combination with recombinant TNFa protein STNFA in ELISA applications.

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

Specifications

• Antigen: TNF alpha
• Applications: ELISA, Western Blot
• Classification: Monoclonal
• Clone: 2TNF-H33
• Concentration: 0.5 mg/mL
• Conjugate: Biotin
• Formulation: PBS with 4% BSA and proprietary preservative
• Gene: Tnf
• Gene Accession No.: P01375
• Gene Alias: APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF α; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFα; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor
• Gene Symbols: Tnf
• Host Species: Mouse
• Immunogen: Recombinant human TNF alpha.
• Purification Method: Protein G
• Quantity: 250 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 7124
• Target Species: Human
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1 κ